PUBLICATION

Origin and role of the cerebrospinal fluid bidirectional flow in the central canal

Authors
Thouvenin, O., Keiser, L., Cantaut-Belarif, Y., Carbo-Tano, M., Verweij, F., Jurisch-Yaksi, N., Bardet, P.L., van Niel, G., Gallaire, F., Wyart, C.
ID
ZDB-PUB-200110-11
Date
2020
Source
eLIFE   9: (Journal)
Registered Authors
Bardet, Pierre-Luc, Jurisch-Yaksi, Nathalie, Wyart, Claire
Keywords
physics of living systems, zebrafish
MeSH Terms
  • Spinal Cord/physiology*
  • Embryo, Nonmammalian/physiology
  • Zebrafish/embryology
  • Zebrafish/physiology
  • Animals
  • Cilia/physiology
  • Cerebral Ventricles/physiology
  • Animals, Genetically Modified
  • Muscle Contraction/physiology
  • Rheology*
  • Embryonic Development
  • Cerebrospinal Fluid/physiology*
  • Biological Transport
  • Green Fluorescent Proteins/metabolism
(all 14)
PubMed
31916933 Full text @ Elife
Abstract
Circulation of the cerebrospinal fluid (CSF) contributes to body axis formation and brain development. Here, we investigated the unexplained origins of the CSF flow bidirectionality in the central canal of the spinal cord of 30 hpf zebrafish embryos and its impact on development. Experiments combined with modeling and simulations demonstrate that the CSF flow is generated locally by caudally-polarized motile cilia along the ventral wall of the central canal. The closed geometry of the canal imposes the average flow rate to be null, explaining the reported bidirectionality. We also demonstrate that at this early stage, motile cilia ensure the proper formation of the central canal. Furthermore, we demonstrate that the bidirectional flow accelerates the transport of particles in the CSF via a coupled convective-diffusive transport process. Our study demonstrates that cilia activity combined with muscle contractions sustain the long-range transport of extracellular lipidic particles, enabling embryonic growth.
Genes / Markers
Figures
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Expression
No data available
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
hsc5TgTransgenic Insertion
    icm13
      Small Deletion
      icm15
        Insertion
        nw2
          Small Deletion
          tm304
            Point Mutation
            tm317b
              Point Mutation
              tp49d
                Point Mutation
                w2
                  Point Mutation
                  1 - 8 of 8
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                  Human Disease / Model
                  No data available
                  Sequence Targeting Reagents
                  Target Reagent Reagent Type
                  foxj1aCRISPR5-foxj1aCRISPR
                  1 - 1 of 1
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                  Fish
                  Antibodies
                  No data available
                  Orthology
                  No data available
                  Engineered Foreign Genes
                  Marker Marker Type Name
                  GFPEFGGFP
                  1 - 1 of 1
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                  Mapping
                  No data available