PUBLICATION

A transcriptomics analysis of the Tbx5 paralogues in zebrafish

Authors

Boyle Anderson, E.A.T., Ho, R.K.

ID

ZDB-PUB-181212-15

Date

2018

Source

PLoS One 13: e0208766 (Journal)

Registered Authors

Ho, Robert K.

Keywords

none

Datasets

GEO:GSE115640

MeSH Terms

Heart/growth & development
Gene Expression Regulation, Developmental
Gene Expression Profiling
Eye/growth & development
Eye/metabolism
Computational Biology
Zebrafish Proteins/genetics
Zebrafish Proteins/metabolism*
T-Box Domain Proteins/genetics
T-Box Domain Proteins/metabolism*
Transcriptome
Animals
Zebrafish
Myocardium/metabolism
Gene Knockdown Techniques

(all 15)

PubMed

30532148 Full text @ PLoS One

Abstract

TBX5 is essential for limb and heart development. Mutations in TBX5 are associated with Holt-Oram syndrome in humans. Due to the teleost specific genome duplication, zebrafish have two copies of TBX5: tbx5a and tbx5b. Both of these genes are expressed in regions of the lateral plate mesoderm and retina. In this study, we perform comparative RNA sequencing analysis on zebrafish embryos during the stages of lateral plate mesoderm migration. This work shows that knockdown of the Tbx5 paralogues results in altered gene expression in many tissues outside of the lateral plate mesoderm, especially in the somitic mesoderm and the intermediate mesoderm. Specifically, knockdown of tbx5b results in changes in somite size, in the differentiation of vasculature progenitors and in later patterning of trunk blood vessels.

Genes / Markers

Figures

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Expression

Gene	Fish	Conditions	Stage	Anatomy	Assay	Figure
aimp1a	AB	standard conditions	20-25 somites	somite	ISH	Fig. S4
aimp1a	AB	standard conditions	20-25 somites	somite	ISH	Fig. 4
aimp1a	AB + MO1-tbx5b	standard conditions	20-25 somites	somite	ISH	Fig. S4
aimp1a	AB + MO1-tbx5b	standard conditions	20-25 somites	somite	ISH	Fig. 4
aimp1a	AB + MO1-tbx5b + MO2-tbx5a	standard conditions	20-25 somites	somite	ISH	Fig. 4
aimp1a	AB + MO1-tbx5b + MO2-tbx5a	standard conditions	20-25 somites	somite	ISH	Fig. S4
aimp1a	AB + MO2-tbx5a	standard conditions	20-25 somites	somite	ISH	Fig. S4
aimp1a	AB + MO2-tbx5a	standard conditions	20-25 somites	somite	ISH	Fig. 4
cox6b1	AB	standard conditions	14-19 somites	pronephros	ISH	Fig. 3
cox6b1	AB + MO1-tbx5b	standard conditions	14-19 somites	pronephros	ISH	Fig. 3

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
AB + MO1-tbx5b	standard conditions	14-19 somites	primordial vasculature sox7 expression decreased amount, abnormal	Fig. 3
AB + MO1-tbx5b	standard conditions	14-19 somites	primordial vasculature sox7 expression spatial pattern, abnormal	Fig. 3
AB + MO1-tbx5b	standard conditions	14-19 somites	pronephros cox6b1 expression decreased amount, abnormal	Fig. 3
AB + MO1-tbx5b	standard conditions	14-19 somites	somite hsp90aa1.2 expression increased amount, abnormal	Fig. S4
AB + MO1-tbx5b	standard conditions	14-19 somites	somite hsp90aa1.2 expression increased amount, abnormal	Fig. 4
AB + MO1-tbx5b	standard conditions	14-19 somites	somite hsp90aa1.2 expression mislocalised, abnormal	Fig. S4
AB + MO1-tbx5b	standard conditions	14-19 somites	somite hsp90aa1.2 expression mislocalised, abnormal	Fig. 4
AB + MO1-tbx5b	standard conditions	14-19 somites	somite mybphb expression increased distribution, abnormal	Fig. S4
AB + MO1-tbx5b	standard conditions	14-19 somites	somite ventral side mybphb expression mislocalised, abnormal	Fig. S4
AB + MO1-tbx5b	standard conditions	14-19 somites	whole organism anterior side phlda2 expression decreased amount, abnormal	Fig. S4

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
ch3		Insertion	tbx5b
y1Tg	Tg(fli1:EGFP)	Transgenic Insertion

Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
tbx5a	MO2-tbx5a	MRPHLNO
tbx5b	CRISPR1-tbx5b	CRISPR
tbx5b	MO1-tbx5b	MRPHLNO

Fish

Fish
AB
AB + MO1-tbx5b
AB + MO1-tbx5b + MO2-tbx5a
AB + MO2-tbx5a
tbx5b^ch3/ch3 (AB)
y1Tg + MO1-tbx5b
y1Tg + MO1-tbx5b + MO2-tbx5a
y1Tg + MO2-tbx5a

Antibodies

Orthology

Engineered Foreign Genes

Marker	Marker Type	Name
EGFP	EFG	EGFP

Mapping

Boyle Anderson et al., 2018 ZDB-PUB-181212-15 PMID:30532148

A transcriptomics analysis of the Tbx5 paralogues in zebrafish

Boyle Anderson et al., 2018

ZDB-PUB-181212-15
PMID:30532148