PUBLICATION

The caudal dorsal artery generates hematopoietic stem and progenitor cells via the endothelial-to-hematopoietic transition in zebrafish

Authors
Zhan, Y., Huang, Y., Chen, J., Cao, Z., He, J., Zhang, J., Huang, H., Ruan, H., Luo, L., Li, L.
ID
ZDB-PUB-180624-3
Date
2018
Source
Journal of genetics and genomics = Yi chuan xue bao : (Journal)
Registered Authors
He, Jianbo, Huang, Honghui, Li, Li, Luo, Lingfei, Ruan, Hua, Zhang, Jingjing
Keywords
Caudal dorsal artery, Circulation, Endothelial-to-hematopoietic transition, Zebrafish
MeSH Terms
none
PubMed
29929848 Full text @ J. Genet. Genomics
Abstract
Zebrafish hematopoietic stem and progenitor cells (HSPCs) originate from the hemogenic endothelium of the ventral wall of the dorsal aorta (DA) through the endothelial-to-hematopoietic transition (EHT) from approximately 30 to 60 hours post fertilization (hpf). However, whether other artery sites can generate HSPCs de novo remains unclear. In this study, using live imaging and lineage tracing, we found that the caudal dorsal artery (CDA) in the caudal hematopoietic tissue directly gave rise to HSPCs through EHT. This process initiated from approximately 60 hpf and terminated at approximately 156 hpf. Compared with that in the DA, fewer EHT events were observed in the CDA. The EHT events in the DA and CDA were similarly regulated by Runx1 but differentially influenced by blood flow (i.e., the EHT frequency in CDA was affected to a lesser extent when circulation was compromised in the tnnt2a-/- mutant). Therefore, the whole artery, including both DA and CDA, was endowed with the ability to produce HSPCs during a much longer time period. Coincidently, the lineage tracing results indicated that adult hematopoietic cells originated from the embryonic endothelium, and those produced later preferentially colonized the adult thymus. Collectively, our study revealed that the CDA serves as an additional source of hematopoiesis, and it shows similar but not identical properties with the DA.
Genes / Markers
Figures
No images available
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
runx1hg1/hg1; s843Tgstandard conditionsPec-fin to Protruding-mouth
tnnt2aswu77/swu77; s843Tgstandard conditionsPrim-25 to Pec-fin
tnnt2aswu77/swu77; s843Tgstandard conditionsPrim-25 to Pec-fin
tnnt2aswu77/swu77; s843Tgstandard conditionsLong-pec to Pec-fin
tnnt2aswu77/swu77; s843Tgstandard conditionsLong-pec to Pec-fin
1 - 5 of 5
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
cq22TgTransgenic Insertion
    cq28TgTransgenic Insertion
      cq52TgTransgenic Insertion
        hg1
          		Point Mutation
          hkz012tTgTransgenic Insertion
            ioz1TgTransgenic Insertion
              la2TgTransgenic Insertion
                s843TgTransgenic Insertion
                  s896TgTransgenic Insertion
                    s928TgTransgenic Insertion
                      1 - 10 of 13
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                      Human Disease / Model
                      No data available
                      Sequence Targeting Reagents
                      No data available
                      Fish
                      Fish
                      cq22Tg
                      cq28Tg; la2Tg
                      runx1hg1/hg1; s843Tg
                      s843Tg
                      s928Tg; zf3387Tg
                      tnnt2aswu77/swu77; s843Tg
                      1 - 6 of 6
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                      Antibodies
                      Name Type Antigen Genes Isotypes Host Organism
                      Ab3-lcp1polyclonalRabbit
                      1 - 1 of 1
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                      Orthology
                      No data available
                      Engineered Foreign Genes
                      Marker Marker Type Name
                      CreEFGCre
                      Dendra2EFGDendra2
                      DsRedEFGDsRed
                      DsRedxEFGDsRedx
                      EGFPEFGEGFP
                      GFPEFGGFP
                      KaedeEFGKaede
                      mCherryEFGmCherry
                      1 - 8 of 8
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                      Mapping
                      No data available
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                      Citation
                      Zhan, Y., Huang, Y., Chen, J., Cao, Z., He, J., Zhang, J., Huang, H., Ruan, H., Luo, L., Li, L. (2018) The caudal dorsal artery generates hematopoietic stem and progenitor cells via the endothelial-to-hematopoietic transition in zebrafish. Journal of genetics and genomics = Yi chuan xue bao. .