PUBLICATION

Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock

Authors

LLeras Forero, L., Narayanan, R., Huitema, L.F.A., VanBergen, M., Apschner, A., Peterson-Maduro, J., Logister, I., Valentin, G., Morelli, L.G., Oates, A., Schulte-Merker, S.

ID

ZDB-PUB-180407-4

Date

2018

Source

eLIFE 7: (Journal)

Registered Authors

Apschner, Alexander, Huitema, Leonie, Logister, Ive, Narayanan, Rachna, Oates, Andrew, Peterson-Maduroe, Josi, Schulte-Merker, Stefan

Keywords

developmental biology, stem cells, zebrafish

MeSH Terms

Notochord/embryology
Notochord/physiology*
Pyrophosphatases/genetics
Pyrophosphatases/metabolism*
Animals
Zebrafish Proteins/genetics
Zebrafish Proteins/metabolism*
Zebrafish/embryology
Zebrafish/genetics
Zebrafish/physiology*
Gene Expression Regulation, Developmental
Body Patterning*
Embryo, Nonmammalian/cytology
Embryo, Nonmammalian/physiology
Animals, Genetically Modified/embryology
Animals, Genetically Modified/genetics
Animals, Genetically Modified/physiology*
Biological Clocks*
Bone and Bones/embryology
Bone and Bones/physiology*
Mutation
Mesoderm/embryology
Mesoderm/physiology

(all 23)

PubMed

29624170 Full text @ Elife

Abstract

Segmentation of the axial skeleton in amniotes depends on the segmentation clock, which patterns the paraxial mesoderm and the sclerotome. While the segmentation clock clearly operates in teleosts, the role of the sclerotome in establishing the axial skeleton is unclear. We severely disrupt zebrafish paraxial segmentation, yet observe a largely normal segmentation process of the chordacentra. We demonstrate that axial entpd5+ notochord sheath cells are responsible for chordacentrum mineralization, and serve as a marker for axial segmentation. While autonomous within the notochord sheath, entpd5 expression and centrum formation show some plasticity and can respond to myotome pattern. These observations reveal for the first time the dynamics of notochord segmentation in a teleost, and are consistent with an autonomous patterning mechanism that is influenced, but not determined by adjacent paraxial mesoderm. This behavior is not consistent with a clock-type mechanism in the notochord.

Genes / Markers

Figures

Show all Figures

Expression

Gene	Fish	Conditions	Stage	Qualifier	Anatomy	Assay	Figure
EGFP	hu7478Tg; nksagff214aGt; nkuasgfp1aTg	standard conditions	Day 6		notochord inner cell	IFL	Fig. 3
en2a	her7^{hu2526/hu2526}; her1^{zf2173/zf2173}	standard conditions	14-19 somites to 20-25 somites		muscle pioneer	ISH	Fig. 1
en2a	her7^{hu2526/hu2526}; tbx6^{sa38869/sa38869}; her1^{zf2173/zf2173}	standard conditions	14-19 somites to 20-25 somites		muscle pioneer	ISH	Fig. 1
en2a	tbx6^{sa38869/sa38869}	standard conditions	14-19 somites to 20-25 somites		muscle pioneer	ISH	Fig. 1
en2a	WT	standard conditions	14-19 somites to 20-25 somites		muscle pioneer	ISH	Fig. 1
her7	her7^{hu2526/hu2526}; her1^{zf2173/zf2173}	standard conditions	5-9 somites to 10-13 somites		segmental plate	ISH	Fig. 1
her7	her7^{hu2526/hu2526}; tbx6^{sa38869/sa38869}; her1^{zf2173/zf2173}	standard conditions	5-9 somites to 10-13 somites	Not Detected	segmental plate anterior side	ISH	Fig. 1
her7	her7^{hu2526/hu2526}; tbx6^{sa38869/sa38869}; her1^{zf2173/zf2173}	standard conditions	5-9 somites to 10-13 somites		segmental plate posterior side	ISH	Fig. 1
her7	her7^{hu2526/hu2526}; tbx6^{sa38869/sa38869}; her1^{zf2173/zf2173}	standard conditions	5-9 somites to 10-13 somites		segmental plate	ISH	Fig. 1
her7	tbx6^{sa38869/sa38869}	standard conditions	5-9 somites to 10-13 somites	Not Detected	segmental plate anterior side	ISH	Fig. 1

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Show

Phenotype

Fish	Conditions	Stage	Phenotype	Figure
her7^{hu2526/hu2526}; her1^{zf2173/zf2173}	standard conditions	5-9 somites to 10-13 somites	segmental plate her7 expression spatial pattern, abnormal	Fig. 1
her7^{hu2526/hu2526}; her1^{zf2173/zf2173}	standard conditions	5-9 somites to 10-13 somites	segmental plate anterior side mespba expression decreased amount, abnormal	Fig. 1
her7^{hu2526/hu2526}; her1^{zf2173/zf2173}	standard conditions	5-9 somites to 10-13 somites	segmental plate anterior side mespba expression spatial pattern, abnormal	Fig. 1
her7^{hu2526/hu2526}; her1^{zf2173/zf2173}	standard conditions	14-19 somites to 20-25 somites	muscle pioneer en2a expression spatial pattern, abnormal	Fig. 1
her7^{hu2526/hu2526}; her1^{zf2173/zf2173}	standard conditions	14-19 somites to 20-25 somites	somite border disorganized, abnormal	Fig. 1
her7^{hu2526/hu2526}; her1^{zf2173/zf2173}	standard conditions	14-19 somites to 20-25 somites	somite border spatial pattern, abnormal	Fig. 1
her7^{hu2526/hu2526}; her1^{zf2173/zf2173}	standard conditions	Prim-25	somite xirp2a expression spatial pattern, abnormal	Fig. 2
her7^{hu2526/hu2526}; her1^{zf2173/zf2173}	standard conditions	Prim-25	somite border disorganized, abnormal	Fig. 2
her7^{hu2526/hu2526}; her1^{zf2173/zf2173}	standard conditions	Prim-25	somite border spatial pattern, abnormal	Fig. 2
her7^{hu2526/hu2526}; her1^{zf2173/zf2173}	standard conditions	Prim-25	somitogenesis process quality, abnormal	Fig. 2

1 - 10 of 47

Show

Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
hu2124		Point Mutation	her1
hu2526		Point Mutation	her7
hu4008Tg	Tg1(Ola.Bglap:EGFP)	Transgenic Insertion
hu5666		Point Mutation	sp7
hu5939Tg	TgBAC(entpd5a:YFP)	Transgenic Insertion
hu6867Tg	TgBAC(entpd5a:Kaede)	Transgenic Insertion
hu7478Tg	TgBAC(entpd5a:KillerRed)	Transgenic Insertion
nksagff214aGt	Gt(GAL4FF)	Transgenic Insertion
nkuasgfp1aTg	Tg(5xUAS:EGFP)	Transgenic Insertion
sa38869		Point Mutation	tbx6

1 - 10 of 16

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Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
her1	TALEN1-her1	TALEN
hes6	TALEN1-hes6	TALEN

Fish

Fish
dlc^tm98/tm98
dld^tr233/tr233
her1^{hu2124/hu2124}
her7^{hu2526/hu2526}
her7^{hu2526/hu2526}; her1^{zf2173/zf2173}
her7^{hu2526/hu2526}; her1^{zf2173/zf2173}; hu6867Tg
her7^{hu2526/hu2526}; tbx6^{sa38869/sa38869}; her1^{zf2173/zf2173}
hes6^{zf2172/zf2172}
hes6^{zf2172/zf2172}; hu5939Tg
hu5939Tg; zf131Tg

1 - 10 of 15

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Antibodies

Orthology

Engineered Foreign Genes

Marker	Marker Type	Name
EGFP	EFG	EGFP
GAL4FF	EFG	GAL4FF
Kaede	EFG	Kaede
KillerRed	EFG	KillerRed
mCherry	EFG	mCherry
YFP	EFG	YFP

Mapping

LLeras Forero et al., 2018 ZDB-PUB-180407-4 PMID:29624170

Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock

LLeras Forero et al., 2018

ZDB-PUB-180407-4
PMID:29624170