PUBLICATION

Notch/Her12 signalling modulates, motile/immotile cilia ratio downstream of Foxj1a in zebrafish left-right organizer.

Authors
Tavares, B., Jacinto, R., Sampaio, P., Pestana, S., Pinto, A., Vaz, A., Roxo-Rosa, M., Gardner, R., Lopes, T., Schilling, B., Henry, I., Saúde, L., Lopes, S.S.
ID
ZDB-PUB-170907-3
Date
2017
Source
eLIFE   6: (Journal)
Registered Authors
Lopes, Susana, Pinto, Andreia
Keywords
developmental biology, stem cells, zebrafish
MeSH Terms
  • Microscopy, Fluorescence
  • Intravital Microscopy
  • Cilia/physiology*
  • Receptors, Notch/metabolism*
  • Animals
  • Signal Transduction
  • Forkhead Transcription Factors/metabolism*
  • Gene Expression Profiling
  • Motion
  • Zebrafish Proteins/metabolism*
  • Zebrafish
  • Basic Helix-Loop-Helix Transcription Factors/metabolism*
(all 12)
PubMed
28875937 Full text @ Elife
Abstract
Foxj1a is necessary and sufficient to specify motile cilia. Using transcriptional studies and slow-scan two-photon live imaging capable of identifying the number of motile and immotile cilia, we now established that the final number of motile cilia depends on Notch signalling (NS). We found that despite all left-right organizer (LRO) cells express foxj1a and the ciliary axonemes of these cells have dynein arms, some cilia remain immotile. We identified that this decision is taken early in development in the Kupffer's Vesicle (KV) precursors the readout being her12 transcription. We demonstrate that overexpression of either her12 or Notch intracellular domain (NICD) increases the number of immotile cilia at the expense of motile cilia, and leads to an accumulation of immotile cilia at the anterior half of the KV. This disrupts the normal fluid flow intensity and pattern, with consequent impact on dand5 expression pattern and left-right (L-R) axis establishment.
Genes / Markers
Figures
Figure Gallery (9 images)
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
s870TgTransgenic Insertion
    tm98
      		Point Mutation
      tr233
        		Point Mutation
        zf417TgTransgenic Insertion
          1 - 4 of 4
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          Human Disease / Model
          No data available
          Sequence Targeting Reagents
          No data available
          Fish
          No data available
          Antibodies
          Orthology
          No data available
          Engineered Foreign Genes
          Marker Marker Type Name
          GFPEFGGFP
          1 - 1 of 1
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          Mapping
          No data available
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          Citation
          Tavares, B., Jacinto, R., Sampaio, P., Pestana, S., Pinto, A., Vaz, A., Roxo-Rosa, M., Gardner, R., Lopes, T., Schilling, B., Henry, I., Saúde, L., Lopes, S.S. (2017) Notch/Her12 signalling modulates, motile/immotile cilia ratio downstream of Foxj1a in zebrafish left-right organizer.. eLIFE. 6.