PUBLICATION

Trim33 / Tif1-γ is essential for macrophage and neutrophil mobilisation to developmental or inflammatory cues

Authors
Demy, D.L., Tauzin, M., Lancino, M., le Cabec, V., Redd, M., Murayama, E., Maridonneau-Parini, I., Trede, N., Herbomel, P.
ID
ZDB-PUB-170721-7
Date
2017
Source
Journal of Cell Science   130(17): 2797-2807 (Journal)
Registered Authors
Demy, Doris-Lou, Herbomel, Philippe, Lancino, Mylène, Murayama, Emi, Redd, Michael, Tauzin, Muriel, Trede, Nick
Keywords
Amoeboid motility, In vivo, Leukocyte recruitment, Macrophages, Neutrophils, Tif1-gamma, Trim33, Zebrafish
MeSH Terms
  • Bacterial Infections/pathology
  • Neutrophils/metabolism*
  • Mice
  • Myeloid Cells/metabolism
  • Cell Movement
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Transcription Factors/deficiency
  • Transcription Factors/genetics
  • Transcription Factors/metabolism*
  • Animals
  • Retina/pathology
  • Bone Marrow Cells/metabolism
  • Microglia/metabolism
  • Central Nervous System/metabolism
  • Central Nervous System/pathology
  • Inflammation/metabolism
  • Inflammation/pathology*
  • Macrophages/metabolism*
  • Mutation/genetics
(all 20)
PubMed
28724755 Full text @ J. Cell Sci.
Abstract
Macrophages infiltrate and establish in developing organs from an early stage, often before these have become vascularized. Similarly, leukocytes, in general, can quickly migrate through tissues to any site of wounding. This unique capacity is rooted in their characteristic amoeboid motility, the genetic basis of which is poorly understood. Trim33 (also known as Tif1-γ), a nuclear protein that associates with specific DNA-binding transcription factors to modulate gene expression, has been found to be mainly involved in hematopoiesis and gene regulation mediated by TGF-β. Here, we have discovered that in Trim33-deficient zebrafish embryos, primitive macrophages are unable to colonize the central nervous system to become microglia. Moreover, both macrophages and neutrophils of Trim33-deficient embryos display a reduced basal mobility within interstitial tissues, and a profound lack of a response to inflammatory recruitment signals, including local bacterial infections. Correlatively, Trim33-deficient mouse bone marrow-derived macrophages display a strongly reduced three-dimensional amoeboid mobility in fibrous collagen gels. The transcriptional regulator Trim33 is thus revealed as being essential for the navigation of macrophages and neutrophils towards developmental or inflammatory cues within vertebrate tissues.
Genes / Markers
Figures
Figure Gallery (11 images) / 2
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
f12TgTransgenic Insertion
    f13TgTransgenic Insertion
      gl25TgTransgenic Insertion
        i114TgTransgenic Insertion
          knu3TgTransgenic Insertion
            nz50TgTransgenic Insertion
              sd2TgTransgenic Insertion
                t30813
                  Unknown
                  tb222b
                    Point Mutation
                    ump2TgTransgenic Insertion
                      1 - 10 of 11
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                      Human Disease / Model
                      No data available
                      Sequence Targeting Reagents
                      Target Reagent Reagent Type
                      trim33MO4-trim33MRPHLNO
                      1 - 1 of 1
                      Show
                      Fish
                      Antibodies
                      No data available
                      Orthology
                      No data available
                      Engineered Foreign Genes
                      Marker Marker Type Name
                      DsRedEFGDsRed
                      DsRed2EFGDsRed2
                      EGFPEFGEGFP
                      GAL4EFGGAL4
                      GAL4FFEFGGAL4FF
                      GFPEFGGFP
                      mCherryEFGmCherry
                      RFPEFGRFP
                      YFPEFGYFP
                      1 - 9 of 9
                      Show
                      Mapping
                      No data available