PUBLICATION

Identification of target genes downstream of Semaphorin6A/PlexinA2 signaling in zebrafish

Authors

Emerson, S.E., St Clair, R.M., Waldron, A.L., Bruno, S.R., Duong, A., Driscoll, H.E., Ballif, B.A., McFarlane, S., Ebert, A.M.

ID

ZDB-PUB-170426-4

Date

2017

Source

Developmental Dynamics : an official publication of the American Association of Anatomists 246(7): 539-549 (Journal)

Registered Authors

Keywords

Retina, eye development, microarray, proliferation, rasl11b, rx3:GFP

Datasets

GEO:GSE86246

MeSH Terms

Gene Expression Regulation, Developmental*/genetics
Retina/cytology
Animals
Stem Cells
Eye/embryology
Eye/growth & development
Zebrafish
Cell Movement
Cell Proliferation
Zebrafish Proteins/metabolism*
Semaphorins/metabolism*
Receptors, Cell Surface/metabolism*
Signal Transduction/physiology*

(all 13)

PubMed

28440030 Full text @ Dev. Dyn.

Abstract

Background Semaphorin (Sema)/Plexin (Plxn) signaling is important for many aspects of neuronal development, however the transcriptional regulation imposed by this signaling pathway is unknown. Previously, we identified an essential role for Sema6A/PlxnA2 signaling in regulating proliferation and cohesion of retinal precursor cells (RPCs) during early eye development. This study used RNA isolated from control, Sema6A-deficient, and PlxnA2-deficient zebrafish embryos in a microarray analysis to identify genes that were differentially expressed when this signaling pathway was disrupted.

Results We uncovered a set of 58 transcripts, and all but one were up-regulated in both sema6A and plxnA2 morphants. We validated gene expression changes in subset of candidates that are suggested to be involved in proliferation, migration or neuronal positioning. We further functionally evaluated one gene, rasl11b, as contributing to disrupted proliferation in sema6A and plxna2 morphants. Our results suggest rasl11b negatively regulates proliferation of RPCs in the developing zebrafish eye.

Conclusions Microarray analysis has generated a resource of target genes downstream of Sema6A/PlxnA2 signaling, which can be further investigated to elucidate the downstream effects of this well-studied neuronal and vascular guidance signaling pathway.

Genes / Markers

Figures

Show all Figures

Expression

Gene	Fish	Conditions	Stage	Qualifier	Anatomy	Assay	Figure
dcdc2b	WT	control	14-19 somites		whole organism	ISH	Fig. 4
dcdc2b	WT	control	14-19 somites		whole organism	RTPCR	Fig. 4
dcdc2b	WT + MO1-plxna2	standard conditions	14-19 somites		whole organism	ISH	Fig. 4
dcdc2b	WT + MO1-plxna2	standard conditions	14-19 somites		whole organism	RTPCR	Fig. 4
dcdc2b	WT + MO1-sema6a	standard conditions	14-19 somites		whole organism	ISH	Fig. 4
dcdc2b	WT + MO1-sema6a	standard conditions	14-19 somites		whole organism	RTPCR	Fig. 4
GFP	zf460Tg	standard conditions	14-19 somites		optic vesicle	IFL	Fig. 1
GFP	zf460Tg + MO1-plxna2	standard conditions	14-19 somites		optic vesicle	IFL	Fig. 1
GFP	zf460Tg + MO1-sema6a	standard conditions	14-19 somites		optic vesicle	IFL	Fig. 1
mmp2	WT	control	14-19 somites	Not Detected	whole organism	ISH	Fig. 4

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
WT + MO1-plxna2	standard conditions	14-19 somites	whole organism dcdc2b expression increased amount, abnormal	Fig. 4
WT + MO1-plxna2	standard conditions	14-19 somites	whole organism dcdc2b expression increased amount, abnormal	Fig. 4
WT + MO1-plxna2	standard conditions	14-19 somites	whole organism mmp2 expression increased amount, abnormal	Fig. 4
WT + MO1-plxna2	standard conditions	14-19 somites	whole organism mmp2 expression increased amount, abnormal	Fig. 4
WT + MO1-plxna2	standard conditions	14-19 somites	whole organism rasl11b expression increased amount, abnormal	Fig. 4
WT + MO1-plxna2	standard conditions	14-19 somites	whole organism rasl11b expression increased amount, abnormal	Fig. 4
WT + MO1-plxna2	standard conditions	14-19 somites	whole organism rbl2 expression increased amount, abnormal	Fig. 4
WT + MO1-plxna2	standard conditions	14-19 somites	whole organism rbl2 expression increased amount, abnormal	Fig. 4
WT + MO1-plxna2	standard conditions	14-19 somites	whole organism shtn1 expression increased amount, abnormal	Fig. 4
WT + MO1-plxna2	standard conditions	14-19 somites	whole organism shtn1 expression increased amount, abnormal	Fig. 4

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
zf460Tg	Tg(Ola.Rx3:GFP)	Transgenic Insertion

Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
plxna2	MO1-plxna2	MRPHLNO
sema6a	MO1-sema6a	MRPHLNO

Fish

Fish
WT
WT + MO1-plxna2
WT + MO1-sema6a
zf460Tg
zf460Tg + MO1-plxna2
zf460Tg + MO1-sema6a

Antibodies

Name	Type	Antigen Genes	Isotypes	Host Organism
Ab36-h3	monoclonal		IgG	Rabbit

Orthology

Engineered Foreign Genes

Marker	Marker Type	Name
GFP	EFG	GFP

Mapping

Emerson et al., 2017 ZDB-PUB-170426-4 PMID:28440030

Identification of target genes downstream of Semaphorin6A/PlexinA2 signaling in zebrafish

Emerson et al., 2017

ZDB-PUB-170426-4
PMID:28440030