PUBLICATION

FGF and canonical Wnt signaling cooperate to induce paraxial mesoderm from tailbud neuromesodermal progenitors through regulation of a two-step EMT

Authors
Goto, H., Kimmey, S.C., Row, R.H., Matus, D.Q., Martin, B.L.
ID
ZDB-PUB-170301-7
Date
2017
Source
Development (Cambridge, England)   144(8): 1412-1424 (Journal)
Registered Authors
Martin, Benjamin, Row, Richard H.
Keywords
EMT, FGF, Wnt, neuromesodermal progenitor, tailbud, zebrafish
MeSH Terms
  • Vimentin/chemistry
  • Vimentin/metabolism
  • Zebrafish Proteins
  • Imaging, Three-Dimensional
  • Epithelial-Mesenchymal Transition*
  • Xenopus laevis/embryology
  • Mesoderm/cytology
  • Mesoderm/embryology*
  • Mesoderm/metabolism
  • T-Box Domain Proteins
  • Amino Acid Sequence
  • Nervous System/cytology*
  • Nervous System/embryology*
  • Animals
  • Stem Cells/cytology*
  • Stem Cells/metabolism
  • Zebrafish/embryology
  • Gastrula/metabolism
  • Tail/embryology*
  • Wnt Signaling Pathway*
  • Fibroblast Growth Factors/metabolism*
(all 21)
PubMed
28242612 Full text @ Development
Abstract
Mesoderm induction begins during gastrulation. Recent evidence from several vertebrate species indicates that mesoderm induction continues after gastrulation in neuromesodermal progenitors (NMPs) within the posteriormost embryonic structure, the tailbud. It is unclear to what extent the molecular mechanisms of mesoderm induction are conserved between gastrula and post-gastrula stages of development. Fibroblast growth factor (FGF) signaling is required for mesoderm induction during gastrulation through positive transcriptional regulation of the T-box transcription factor brachyury We find in zebrafish that FGF is continuously required for paraxial mesoderm (PM) induction in post-gastrula NMPs. FGF signaling represses the NMP markers brachyury (ntla) and sox2 through regulation of tbx16 and msgn1, thereby committing cells to a PM fate. FGF-mediated PM induction in NMPs functions in tight coordination with canonical Wnt signaling during the epithelial to mesenchymal transition (EMT) from NMP to mesodermal progenitor. Wnt signaling initiates EMT, whereas FGF signaling terminates this event. Our results indicate that germ layer induction in the zebrafish tailbud is not a simple continuation of gastrulation events.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b104
    Indel
    pd1TgTransgenic Insertion
      pd3TgTransgenic Insertion
        sbu101TgTransgenic Insertion
          sbu103TgTransgenic Insertion
            sbu104TgTransgenic Insertion
              w74TgTransgenic Insertion
                w130TgTransgenic Insertion
                  1 - 8 of 8
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                  Human Disease / Model
                  No data available
                  Sequence Targeting Reagents
                  No data available
                  Fish
                  Antibodies
                  No data available
                  Orthology
                  No data available
                  Engineered Foreign Genes
                  Marker Marker Type Name
                  DsRedEFGDsRed
                  EGFPEFGEGFP
                  KikGREFGKikGR
                  mCherryEFGmCherry
                  TFPEFGTFP
                  1 - 5 of 5
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                  Mapping
                  No data available