PUBLICATION

Radial glia regulate vascular patterning around the developing spinal cord

Authors
Matsuoka, R.L., Marass, M., Avdesh, A., Helker, C.S., Maischein, H.M., Grosse, A.S., Kaur, H., Lawson, N.D., Herzog, W., Stainier, D.Y.
ID
ZDB-PUB-161118-6
Date
2016
Source
eLIFE   5: (Journal)
Registered Authors
Helker, Christian, Herzog, Wiebke, Lawson, Nathan, Maischein, Hans-Martin, Matsuoka, Ryota, Stainier, Didier
Keywords
developmental biology, neuroscience, stem cells, zebrafish
MeSH Terms
  • Neuroglia/metabolism
  • Signal Transduction/genetics
  • Mosaicism
  • Zebrafish Proteins/genetics*
  • Animals
  • Endothelial Cells/metabolism
  • Vascular Endothelial Growth Factor Receptor-1/genetics*
  • Vascular Endothelial Growth Factor A/genetics*
  • Organogenesis/genetics*
  • Gene Expression Regulation
  • Cell Differentiation/genetics*
  • Spinal Cord/blood supply
  • Spinal Cord/growth & development
  • Spinal Cord/metabolism*
  • Neural Stem Cells/metabolism
  • Zebrafish/genetics
  • Zebrafish/growth & development
  • Blood Vessels/growth & development
  • Blood Vessels/metabolism
(all 19)
PubMed
27852438 Full text @ Elife
Abstract
Vascular networks surrounding individual organs are important for their development, maintenance, and function; however, how these networks are assembled remains poorly understood. Here we show that CNS progenitors, referred to as radial glia, modulate vascular patterning around the spinal cord by acting as negative regulators. We found that radial glia ablation in zebrafish embryos leads to excessive sprouting of the trunk vessels around the spinal cord, and exclusively those of venous identity. Mechanistically, we determined that radial glia control this process via the Vegf decoy receptor sFlt1: sflt1 mutants exhibit the venous over-sprouting observed in radial glia-ablated larvae, and sFlt1 overexpression rescues it. Genetic mosaic analyses show that sFlt1 function in trunk endothelial cells can limit their over-sprouting. Together, our findings identify CNS-resident progenitors as critical angiogenic regulators that determine the precise patterning of the vasculature around the spinal cord, providing novel insights into vascular network formation around developing organs.
Genes / Markers
Figures
Figure Gallery (12 images) / 2
Show all Figures
Expression
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay Figure
EGFPba2Tg; s896Tgchemical treatment by environment: CyclopamineDay 6IFL
EGFPba2Tg; s896TgcontrolDay 6IFL
EGFPbns80Tg; c264Tg; s843Tg; s995Tgchemical ablation: radial glial cell, heat shock, chemical treatment by environment: metronidazoleDay 6IFL
EGFPbns82Tg; c264Tg; s843Tg; s995Tgchemical ablation: radial glial cell, chemical treatment by environment: metronidazoleDay 6IFL
EGFPbns82Tg; c264Tg; s843Tg; s995Tgchemical ablation: radial glial cell, heat shock, chemical treatment by environment: metronidazoleDay 6IFL
EGFPbns82Tg; c264Tg; s843Tg; s995Tgchemical ablation: radial glial cell, chemical treatment by environment: metronidazoleDay 6IFL
EGFPbns82Tg; c264Tg; s843Tg; s995Tgchemical ablation: radial glial cell, heat shock, chemical treatment by environment: metronidazoleDay 6IFL
EGFPbns82Tg; c264Tg; s843Tg; s995Tgchemical ablation: radial glial cell, chemical treatment by environment: metronidazoleDay 6IFL
EGFPbns82Tg; c264Tg; s843Tg; s995Tgchemical ablation: radial glial cell, heat shock, chemical treatment by environment: metronidazoleDay 6IFL
EGFPbns82Tg; c264Tg; s843Tg; s995Tgchemical ablation: radial glial cell, heat shock, chemical treatment by environment: metronidazoleDay 6IFL
1 - 10 of 115
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Phenotype
Fish Conditions Stage Phenotype Figure
ba2Tg; s896Tgchemical treatment by environment: CyclopamineDay 6
bns80Tg; c264Tg; s843Tg; s995Tgchemical ablation: radial glial cell, heat shock, chemical treatment by environment: metronidazoleDay 6
bns80Tg; c264Tg; s843Tg; s995Tgchemical ablation: radial glial cell, heat shock, chemical treatment by environment: metronidazoleDay 6
bns82Tg; c264Tg; s843Tg; s995Tgchemical ablation: radial glial cell, chemical treatment by environment: metronidazoleDay 6
bns82Tg; c264Tg; s843Tg; s995Tgchemical ablation: radial glial cell, chemical treatment by environment: metronidazoleDay 6
bns82Tg; c264Tg; s843Tg; s995Tgchemical ablation: radial glial cell, chemical treatment by environment: metronidazoleDay 6
bns82Tg; c264Tg; s843Tg; s995Tgchemical ablation: radial glial cell, heat shock, chemical treatment by environment: metronidazoleDay 6
bns82Tg; c264Tg; s843Tg; s995Tgchemical ablation: radial glial cell, heat shock, chemical treatment by environment: metronidazoleDay 6
bns82Tg; c264Tg; s843Tg; s995Tgchemical ablation: radial glial cell, heat shock, chemical treatment by environment: metronidazoleDay 6
c264Tg; hu4624Tg; psi1Tgchemical ablation: neuron, chemical treatment by environment: metronidazoleProtruding-mouth
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
ba2TgTransgenic Insertion
    bns29
      Indel
      bns80TgTransgenic Insertion
        bns82TgTransgenic Insertion
          c264TgTransgenic Insertion
            ci1TgTransgenic Insertion
              fh390
                Unknown
                fr14TgTransgenic Insertion
                  hu4624TgTransgenic Insertion
                    hu5333TgTransgenic Insertion
                      1 - 10 of 25
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                      Human Disease / Model
                      No data available
                      Sequence Targeting Reagents
                      Target Reagent Reagent Type
                      flt1CRISPR1-flt1CRISPR
                      flt1CRISPR2-flt1CRISPR
                      1 - 2 of 2
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                      Fish
                      Antibodies
                      Orthology
                      No data available
                      Engineered Foreign Genes
                      Marker Marker Type Name
                      CeruleanEFGCerulean
                      EGFPEFGEGFP
                      GAL4EFGGAL4
                      GAL4FFEFGGAL4FF
                      GFPEFGGFP
                      mCherryEFGmCherry
                      NTREFGNTR
                      RFPEFGRFP
                      TagBFPEFGTagBFP
                      YFPEFGYFP
                      1 - 10 of 10
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                      Mapping
                      No data available