PUBLICATION

Myosin phosphatase Fine-tunes Zebrafish Motoneuron Position during Axonogenesis

Authors
Bremer, J., Granato, M.
ID
ZDB-PUB-161118-2
Date
2016
Source
PLoS Genetics   12: e1006440 (Journal)
Registered Authors
Granato, Michael
Keywords
Axons, Embryos, Myosins, Notochords, Spinal cord, Muscle cells, Motor neurons, Zebrafish
MeSH Terms
  • Muscle Cells/cytology
  • Muscle Cells/metabolism
  • Myosin-Light-Chain Phosphatase/biosynthesis
  • Myosin-Light-Chain Phosphatase/genetics*
  • Embryonic Development/genetics*
  • Notochord/growth & development
  • Zebrafish/genetics
  • Zebrafish/growth & development
  • Neurogenesis/genetics*
  • Animals
  • Motor Neurons/cytology
  • Motor Neurons/metabolism
  • Axons*
  • Spinal Cord/growth & development
  • Gene Expression Regulation, Developmental
(all 15)
PubMed
27855159 Full text @ PLoS Genet.
Abstract
During embryogenesis the spinal cord shifts position along the anterior-posterior axis relative to adjacent tissues. How motor neurons whose cell bodies are located in the spinal cord while their axons reside in adjacent tissues compensate for such tissue shift is not well understood. Using live cell imaging in zebrafish, we show that as motor axons exit from the spinal cord and extend through extracellular matrix produced by adjacent notochord cells, these cells shift several cell diameters caudally. Despite this pronounced shift, individual motoneuron cell bodies stay aligned with their extending axons. We find that this alignment requires myosin phosphatase activity within motoneurons, and that mutations in the myosin phosphatase subunit mypt1 increase myosin phosphorylation causing a displacement between motoneuron cell bodies and their axons. Thus, we demonstrate that spinal motoneurons fine-tune their position during axonogenesis and we identify the myosin II regulatory network as a key regulator.
Genes / Markers
Figures
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Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
ml2TgTransgenic Insertion
    nkuasgfp1aTgTransgenic Insertion
      p82emcf
        Point Mutation
        p187TgTransgenic Insertion
          p188TgTransgenic Insertion
            smb1226TgTransgenic Insertion
              1 - 6 of 6
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              Human Disease / Model
              No data available
              Sequence Targeting Reagents
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              Fish
              Antibodies
              Orthology
              No data available
              Engineered Foreign Genes
              Marker Marker Type Name
              EGFPEFGEGFP
              GAL4FFEFGGAL4FF
              GFPEFGGFP
              mKate2EFGmKate2
              1 - 4 of 4
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              Mapping
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