PUBLICATION

Loss of laminin alpha 1 results in multiple structural defects and divergent effects on adhesion during vertebrate optic cup morphogenesis

Authors

Bryan, C.D., Chien, C.B., Kwan, K.M.

ID

ZDB-PUB-160625-6

Date

2016

Source

Developmental Biology 416(2): 324-37 (Journal)

Registered Authors

Chien, Chi-Bin, Kwan, Kristen

Keywords

adhesion, cell polarity, eye morphogenesis, laminin, lens, retina

MeSH Terms

Retinal Pigment Epithelium/cytology
Retinal Pigment Epithelium/embryology
Cell Polarity
Zebrafish Proteins/deficiency
Zebrafish Proteins/genetics
Zebrafish Proteins/physiology*
Microscopy, Confocal
Extracellular Matrix/physiology
Laminin/deficiency
Laminin/genetics
Laminin/physiology*
Cell Movement
Time-Lapse Imaging
Animals
Eye Proteins/genetics
Eye Proteins/physiology*
Luminescent Proteins/analysis
Luminescent Proteins/genetics
Retinal Ganglion Cells/cytology
Lens, Crystalline/embryology*
Retina/cytology
Retina/embryology*
Organogenesis
Focal Adhesions
Zebrafish/embryology*

(all 25)

PubMed

27339294 Full text @ Dev. Biol.

Abstract

The vertebrate eye forms via a complex set of morphogenetic events. The optic vesicle evaginates and undergoes transformative shape changes to form the optic cup, in which neural retina and retinal pigmented epithelium enwrap the lens. It has long been known that a complex, glycoprotein-rich extracellular matrix layer surrounds the developing optic cup throughout the process, yet the functions of the matrix and its specific molecular components have remained unclear. Previous work established a role for laminin extracellular matrix in particular steps of eye development, including optic vesicle evagination, lens differentiation, and retinal ganglion cell polarization, yet it is unknown what role laminin might play in the early process of optic cup formation subsequent to the initial step of optic vesicle evagination. Here, we use the zebrafish lama1 mutant (lama1(UW1)) to determine the function of laminin during optic cup morphogenesis. Using live imaging, we find, surprisingly, that loss of laminin leads to divergent effects on focal adhesion assembly in a spatiotemporally-specific manner, and that laminin is required for multiple steps of optic cup morphogenesis, including optic stalk constriction, invagination, and formation of a spherical lens. Laminin is not required for single cell behaviors and changes in cell shape. Rather, in lama1(UW1) mutants, loss of epithelial polarity and altered adhesion lead to defective tissue architecture and formation of a disorganized retina. These results demonstrate that the laminin extracellular matrix plays multiple critical roles regulating adhesion and polarity to establish and maintain tissue structure during optic cup morphogenesis.

Genes / Markers

Figures

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Expression

Phenotype

Fish	Conditions	Stage	Phenotype	Figure
lama1^uw1/uw1	standard conditions	5-9 somites	optic vesicle ab1-lama1 labeling absent, abnormal	Fig. 1
lama1^uw1/uw1	standard conditions	10-13 somites	cell motility involved in camera-type eye morphogenesis disrupted, abnormal	Fig. 1
lama1^uw1/uw1	standard conditions	10-13 somites	optic cup morphogenesis involved in camera-type eye development disrupted, abnormal	Fig. 1
lama1^uw1/uw1	standard conditions	10-13 somites	optic furrow malformed, abnormal	Fig. 1
lama1^uw1/uw1	standard conditions	10-13 somites	presumptive neural retina cell decreased length, abnormal	Fig. 5
lama1^uw1/uw1	standard conditions	14-19 somites	optic fissure focal adhesion decreased amount, abnormal	Fig. 3
lama1^uw1/uw1	standard conditions	Prim-5	cell motility involved in camera-type eye morphogenesis disrupted, abnormal	Fig. 1
lama1^uw1/uw1	standard conditions	Prim-5	establishment of epithelial cell apical/basal polarity involved in camera-type eye morphogenesis disrupted, abnormal	Fig. 6
lama1^uw1/uw1	standard conditions	Prim-5	lens development in camera-type eye process quality, abnormal	Fig. 3
lama1^uw1/uw1	standard conditions	Prim-5	lens ovate, abnormal	Fig. 1

1 - 10 of 24

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
uw1		Point Mutation	lama1

Human Disease / Model

Sequence Targeting Reagents

Fish

Fish
lama1^uw1/uw1
WT

Antibodies

Orthology

Engineered Foreign Genes

Mapping

Bryan et al., 2016 ZDB-PUB-160625-6 PMID:27339294

Loss of laminin alpha 1 results in multiple structural defects and divergent effects on adhesion during vertebrate optic cup morphogenesis

Bryan et al., 2016

ZDB-PUB-160625-6
PMID:27339294