PUBLICATION

An Fgf-Shh signaling hierarchy regulates early specification of the zebrafish skull

Authors
McCarthy, N., Sidik, A., Bertrand, J.Y., Eberhart, J.K.
ID
ZDB-PUB-160411-1
Date
2016
Source
Developmental Biology   415(2): 261-77 (Journal)
Registered Authors
Bertrand, Julien, Eberhart, Johann, McCarthy, Neil
Keywords
Fgf, Shh, craniofacial development, head mesoderm, zebrafish
MeSH Terms
  • Skull/embryology*
  • Skull/metabolism
  • Animals
  • Mesoderm/embryology
  • Mesoderm/metabolism
  • Signal Transduction*
  • Fibroblast Growth Factors/deficiency
  • Fibroblast Growth Factors/genetics
  • Fibroblast Growth Factors/physiology*
  • Zebrafish Proteins/deficiency
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology*
  • Cell Differentiation
  • Hedgehog Proteins/genetics
  • Hedgehog Proteins/physiology*
  • Glucuronosyltransferase/genetics
  • Glucuronosyltransferase/physiology*
  • Fibroblast Growth Factor 3/deficiency
  • Fibroblast Growth Factor 3/genetics
  • Fibroblast Growth Factor 3/physiology*
  • Gene Expression Regulation, Developmental
  • Zebrafish/embryology*
  • Zebrafish/genetics
(all 23)
PubMed
27060628 Full text @ Dev. Biol.
Abstract
The neurocranium generates most of the craniofacial skeleton and consists of prechordal and postchordal regions. Although development of the prechordal is well studied, little is known of the postchordal region. Here we characterize a signaling hierarchy necessary for postchordal neurocranial development involving Fibroblast growth factor (Fgf) signaling for early specification of mesodermally-derived progenitor cells. The expression of hyaluron synthetase 2 (has2) in the cephalic mesoderm requires Fgf signaling and Has2 function, in turn, is required for postchordal neurocranial development. While Hedgehog (Hh)-deficient embryos also lack a postchordal neurocranium, this appears primarily due to a later defect in chondrocyte differentiation. Inhibitor studies demonstrate that postchordal neurocranial development requires early Fgf and later Hh signaling. Collectively, our results provide a mechanistic understanding of early postchordal neurocranial development and demonstrate a hierarchy of signaling between Fgf and Hh in the development of this structure.
Genes / Markers
Figures
Figure Gallery (21 images) / 3
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b195
    Insertion
    b577
      Point Mutation
      cz1701TgTransgenic Insertion
        el2TgTransgenic Insertion
          t24149
            Point Mutation
            ti282a
              Point Mutation
              tud103TgTransgenic Insertion
                ug101TgTransgenic Insertion
                  zf384TgTransgenic Insertion
                    zf393TgTransgenic Insertion
                      1 - 10 of 10
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                      Human Disease / Model
                      No data available
                      Sequence Targeting Reagents
                      Target Reagent Reagent Type
                      dlx3bMO2-dlx3bMRPHLNO
                      fgf3MO2-fgf3MRPHLNO
                      fgf3MO3-fgf3MRPHLNO
                      fgf8aMO1-fgf8aMRPHLNO
                      fgf8aMO2-fgf8aMRPHLNO
                      fgfr3MO1-fgfr3MRPHLNO
                      foxi1MO2-foxi1MRPHLNO
                      has2MO1-has2MRPHLNO
                      has2MO2-has2MRPHLNO
                      1 - 9 of 9
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                      Fish
                      Antibodies
                      No data available
                      Orthology
                      No data available
                      Engineered Foreign Genes
                      Marker Marker Type Name
                      CreEFGCre
                      DsRedEFGDsRed
                      DsRed2EFGDsRed2
                      EGFPEFGEGFP
                      KaedeEFGKaede
                      KikGREFGKikGR
                      mCherryEFGmCherry
                      1 - 7 of 7
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                      Mapping
                      No data available