PUBLICATION

An Fgf-Shh signaling hierarchy regulates early specification of the zebrafish skull

Authors

McCarthy, N., Sidik, A., Bertrand, J.Y., Eberhart, J.K.

ID

ZDB-PUB-160411-1

Date

2016

Source

Developmental Biology 415(2): 261-77 (Journal)

Registered Authors

Bertrand, Julien, Eberhart, Johann, McCarthy, Neil

Keywords

Fgf, Shh, craniofacial development, head mesoderm, zebrafish

MeSH Terms

Glucuronosyltransferase/genetics
Glucuronosyltransferase/physiology*
Mesoderm/embryology
Mesoderm/metabolism
Zebrafish/embryology*
Zebrafish/genetics
Skull/embryology*
Skull/metabolism
Fibroblast Growth Factor 3/deficiency
Fibroblast Growth Factor 3/genetics
Fibroblast Growth Factor 3/physiology*
Hedgehog Proteins/genetics
Hedgehog Proteins/physiology*
Zebrafish Proteins/deficiency
Zebrafish Proteins/genetics
Zebrafish Proteins/physiology*
Fibroblast Growth Factors/deficiency
Fibroblast Growth Factors/genetics
Fibroblast Growth Factors/physiology*
Signal Transduction*
Cell Differentiation
Animals
Gene Expression Regulation, Developmental

(all 23)

PubMed

27060628 Full text @ Dev. Biol.

Abstract

The neurocranium generates most of the craniofacial skeleton and consists of prechordal and postchordal regions. Although development of the prechordal is well studied, little is known of the postchordal region. Here we characterize a signaling hierarchy necessary for postchordal neurocranial development involving Fibroblast growth factor (Fgf) signaling for early specification of mesodermally-derived progenitor cells. The expression of hyaluron synthetase 2 (has2) in the cephalic mesoderm requires Fgf signaling and Has2 function, in turn, is required for postchordal neurocranial development. While Hedgehog (Hh)-deficient embryos also lack a postchordal neurocranium, this appears primarily due to a later defect in chondrocyte differentiation. Inhibitor studies demonstrate that postchordal neurocranial development requires early Fgf and later Hh signaling. Collectively, our results provide a mechanistic understanding of early postchordal neurocranial development and demonstrate a hierarchy of signaling between Fgf and Hh in the development of this structure.

Genes / Markers

Figures

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Expression

Gene	Fish	Conditions	Stage	Qualifier	Anatomy	Assay	Figure
col2a1a	AB	control	Prim-5		chondrocyte	ISH	Fig. 11
col2a1a	AB	control	Prim-5		chondrocyte	ISH	Fig. 10
col2a1a	AB	control	Prim-5		chondrocyte	ISH	Fig. 5
col2a1a	AB	chemical treatment: Cyclopamine	Prim-5		chondrocyte	ISH	Fig. 11
col2a1a	AB	control	Prim-5		chondrocyte	ISH	Fig. 9
col2a1a	AB + MO2-fgf3 + MO3-fgf3	control	Prim-5		chondrocyte	ISH	Fig. 5
col2a1a	fgf8a^{ti282a/ti282a}	control	Prim-5		chondrocyte	ISH	Fig. 5
col2a1a	fgf8a^{ti282a/ti282a} + MO2-fgf3 + MO3-fgf3	control	Prim-5	Not Detected	chondrocyte	ISH	Fig. 5
col2a1a	smo^b577/b577	control	Prim-5	Not Detected	chondrocyte	ISH	Fig. 10
col2a1a	tbxta^b195/b195	control	Prim-5		chondrocyte	ISH	Fig. 9

1 - 10 of 50

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
AB	chemical treatment: Cyclopamine	Prim-5	chondroblast sox9a expression decreased amount, abnormal	Fig. 11
AB	chemical treatment: Cyclopamine	Prim-5	chondrocyte col2a1a expression decreased amount, abnormal	Fig. 11
AB	chemical treatment: Cyclopamine	Prim-5	neurocranium posterior region sox9a expression decreased amount, abnormal	Fig. 11
fgf3^t24149/+; fgf8a^{ti282a/ti282a}	standard conditions	Day 5	anterior basicapsular commissure absent, abnormal	Fig. 1
fgf3^t24149/+; fgf8a^{ti282a/ti282a}	standard conditions	Day 5	neurocranium posterior region morphology, abnormal	Fig. 1
fgf3^t24149/+; fgf8a^{ti282a/ti282a}	standard conditions	Day 5	parachordal cartilage absent, abnormal	Fig. 1
fgf3^{t24149/t24149}; fgf8a^{ti282a/ti282a}	control	Prim-5	neurocranium has2 expression absent, abnormal	Fig. 7
fgf3^{t24149/t24149}; fgf8a^{ti282a/ti282a}	standard conditions	Day 5	anterior basicapsular commissure absent, abnormal	Fig. 1
fgf3^{t24149/t24149}; fgf8a^{ti282a/ti282a}	standard conditions	Day 5	lateral basicapsular commissure absent, abnormal	Fig. 1
fgf3^{t24149/t24149}; fgf8a^{ti282a/ti282a}	standard conditions	Day 5	neurocranium posterior region absent, abnormal	Fig. 1

1 - 10 of 24

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
b195		Insertion	tbxta
b577		Point Mutation	smo
cz1701Tg	Tg(-3.5ubb:LOXP-EGFP-LOXP-mCherry)	Transgenic Insertion
el2Tg	Tg(sox10:KikGR)	Transgenic Insertion
t24149		Point Mutation	fgf3
ti282a		Point Mutation	fgf8a
tud103Tg	Tg(Xla.Eef1a1:LOXP-DsRed2-LOXP-EGFP)	Transgenic Insertion
ug101Tg	Tg(drl:Cre-ERT2)	Transgenic Insertion
zf384Tg	Tg(Mmu.Sox10-Mmu.Fos:Cre)	Transgenic Insertion
zf393Tg	Tg(sox10:Kaede)	Transgenic Insertion

1 - 10 of 10

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Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
dlx3b	MO2-dlx3b	MRPHLNO
fgf3	MO2-fgf3	MRPHLNO
fgf3	MO3-fgf3	MRPHLNO
fgf8a	MO1-fgf8a	MRPHLNO
fgf8a	MO2-fgf8a	MRPHLNO
fgfr3	MO1-fgfr3	MRPHLNO
foxi1	MO2-foxi1	MRPHLNO
has2	MO1-has2	MRPHLNO
has2	MO2-has2	MRPHLNO

1 - 9 of 9

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Fish

Fish
AB
AB + MO2-fgf3 + MO3-fgf3
cz1701Tg; ug101Tg
el2Tg
fgf3^t24149/+; fgf8a^{ti282a/ti282a}
fgf3^{t24149/t24149}; fgf8a^{ti282a/ti282a}
fgf8a^ti282a/+
fgf8a^{ti282a/ti282a}
fgf8a^{ti282a/ti282a} + MO1-has2 + MO2-has2
fgf8a^{ti282a/ti282a} + MO2-fgf3 + MO3-fgf3

1 - 10 of 14

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Antibodies

Orthology

Engineered Foreign Genes

Marker	Marker Type	Name
Cre	EFG	Cre
DsRed	EFG	DsRed
DsRed2	EFG	DsRed2
EGFP	EFG	EGFP
Kaede	EFG	Kaede
KikGR	EFG	KikGR
mCherry	EFG	mCherry

Mapping

McCarthy et al., 2016 ZDB-PUB-160411-1 PMID:27060628

An Fgf-Shh signaling hierarchy regulates early specification of the zebrafish skull

McCarthy et al., 2016

ZDB-PUB-160411-1
PMID:27060628