PUBLICATION

Two developmentally distinct populations of neural crest cells contribute to the zebrafish heart

Authors
Cavanaugh, A.M., Huang, J., Chen, J.N.
ID
ZDB-PUB-150619-1
Date
2015
Source
Developmental Biology   404(2): 103-12 (Journal)
Registered Authors
Chen, Jau-Nian, Huang, Jie
Keywords
none
MeSH Terms
  • Aorta/cytology
  • Myocytes, Cardiac/cytology
  • Cell Movement
  • Neural Crest/cytology*
  • Cell Differentiation
  • Fibroblast Growth Factors/antagonists & inhibitors*
  • Animals
  • Coronary Vessels/cytology
  • Coronary Vessels/embryology
  • Heart Defects, Congenital/embryology*
  • Zebrafish/embryology*
  • Animals, Genetically Modified
  • Heart/embryology*
(all 13)
PubMed
26086691 Full text @ Dev. Biol.
Abstract
Cardiac neural crest cells are essential for outflow tract remodeling in animals with divided systemic and pulmonary circulatory systems, but their contributions to cardiac development in animals with a single-loop circulatory system are less clear. Here we genetically labeled neural crest cells and examined their contribution to the developing zebrafish heart. We identified two populations of neural crest cells that contribute to distinct compartments of zebrafish cardiovascular system at different developmental stages. A stream of neural crest cells migrating through pharyngeal arches 1 and 2 integrates into the myocardium of the primitive heart tube between 24 and 30hours post fertilization and gives rise to cardiomyocytes. A second wave of neural crest cells migrating along aortic arch 6 envelops the endothelium of the ventral aorta and invades the bulbus arteriosus after three days of development. Interestingly, while inhibition of FGF signaling has no effect on the integration of neural crest cells to the primitive heart tube, it prevents these cells from contributing to the outflow tract, demonstrating disparate responses of neural crest cells to FGF signaling. Furthermore, neural crest ablation in zebrafish leads to multiple cardiac defects, including reduced heart rate, defective myocardial maturation and a failure to recruit progenitor cells from the second heart field. These findings add to our understanding of the contribution of neural crest cells to the developing heart and provide insights into the requirement for these cells in cardiac maturation.
Genes / Markers
Figures
Figure Gallery (8 images)
Show all Figures
Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
c264TgTransgenic Insertion
    chb2TgTransgenic Insertion
      cz1701TgTransgenic Insertion
        la116TgTransgenic Insertion
          la2326TgTransgenic Insertion
            sd22TgTransgenic Insertion
              1 - 6 of 6
              Show
              Human Disease / Model
              No data available
              Sequence Targeting Reagents
              No data available
              Fish
              No data available
              Antibodies
              Name Type Antigen Genes Isotypes Host Organism
              zn-8monoclonalIgG1Mouse
              1 - 1 of 1
              Show
              Orthology
              No data available
              Engineered Foreign Genes
              Marker Marker Type Name
              CreEFGCre
              EGFPEFGEGFP
              GAL4EFGGAL4
              GFPEFGGFP
              KaedeEFGKaede
              mCherryEFGmCherry
              NTREFGNTR
              1 - 7 of 7
              Show
              Mapping
              No data available
              Your Input Welcome
              We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
              Citation
              Cavanaugh, A.M., Huang, J., Chen, J.N. (2015) Two developmentally distinct populations of neural crest cells contribute to the zebrafish heart. Developmental Biology. 404(2):103-12.