PUBLICATION

ELABELA: A Hormone Essential for Heart Development Signals via the Apelin Receptor

Authors
Chng, S.C., Ho, L., Tian, J., and Reversade, B.
ID
ZDB-PUB-140106-3
Date
2013
Source
Developmental Cell   27(6): 672-680 (Journal)
Registered Authors
Chng, Serene, Ho, Lena, REVERSADE, Bruno, Tian, Jing
Keywords
none
MeSH Terms
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish/metabolism
  • Phenotype
  • Gene Expression Regulation, Developmental*
  • Zinc Fingers
  • Animals, Genetically Modified
  • Transcription Factors/genetics
  • Transcription Factors/metabolism*
  • Receptors, G-Protein-Coupled/genetics
  • Receptors, G-Protein-Coupled/metabolism*
  • Cell Differentiation
  • Sequence Homology, Amino Acid
  • Real-Time Polymerase Chain Reaction
  • Humans
  • Animals
  • RNA, Messenger/genetics
  • Blotting, Western
  • Gene Frequency
  • Amino Acid Sequence
  • Molecular Sequence Data
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Peptide Hormones/genetics
  • Peptide Hormones/metabolism*
  • Endoderm
  • Heart/embryology*
  • Mutagenesis, Site-Directed
(all 31)
PubMed
24316148 Full text @ Dev. Cell
Abstract

We report here the discovery and characterization of a gene, ELABELA (ELA), encoding a conserved hormone of 32 amino acids. Present in human embryonic stem cells, ELA is expressed at the onset of zebrafish zygotic transcription and is ubiquitous in the naive ectodermal cells of the embryo. Using zinc-finger-nuclease-mediated gene inactivation in zebrafish, we created an allelic series of ela mutants. ela null embryos have impaired endoderm differentiation potential marked by reduced gata5 and sox17 expression. Loss of Ela causes embryos to develop with a rudimentary heart or no heart at all, surprisingly phenocopying the loss of the apelin receptor (aplnr), which we show serves as Ela's cognate G protein-coupled receptor. Our results reveal the existence of a peptide hormone, ELA, which, together with APLNR, forms an essential signaling axis for early cardiovascular development.

Genes / Markers
Figures
Figure Gallery (4 images)
Show all Figures
Expression
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay Figure
apelaWTstandard conditions5-9 somitesISH
apelaWTstandard conditionsPrim-5ISH
apelaWTstandard conditionsSphereISH
apelaWTstandard conditionsBudISH
apelaWTstandard conditions90%-epibolyISH
apelaWTstandard conditions50%-epibolyISH
apelaWTstandard conditions1-cellISH
apelaWTstandard conditionsSphere to Long-pecRTPCR
aplnraapelazf439/zf439standard conditionsShieldISH
aplnraWTstandard conditionsShieldISH
1 - 10 of 32
Show
Phenotype
Fish Conditions Stage Phenotype Figure
WT + MO1-aplnra + MO1-aplnrbstandard conditionsDay 6
WT + MO1-aplnra + MO1-aplnrbstandard conditionsDay 6
WT + MO1-aplnra + MO1-aplnrbstandard conditionsDay 6
WT + MO1-aplnra + MO1-aplnrbstandard conditionsDay 6
apelazf438/zf438standard conditionsPrim-5
apelazf438/zf438standard conditionsPrim-5
apelazf438/zf438standard conditionsPrim-5
apelazf438/zf438standard conditionsPrim-5
apelazf438/zf438standard conditionsPrim-5
apelazf438/zf438standard conditionsPrim-5
1 - 10 of 34
Show
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
zf438
    		Small Deletion
    zf439
      		Small Deletion
      zf440
        		Small Deletion
        1 - 3 of 3
        Show
        Human Disease / Model
        No data available
        Sequence Targeting Reagents
        Target Reagent Reagent Type
        aplnraMO1-aplnraMRPHLNO
        aplnrbMO1-aplnrbMRPHLNO
        1 - 2 of 2
        Show
        Fish
        Fish
        apelazf438/zf438
        apelazf439/zf439
        apelazf440/zf440
        WT
        WT + MO1-aplnra + MO1-aplnrb
        1 - 5 of 5
        Show
        Antibodies
        No data available
        Orthology
        No data available
        Engineered Foreign Genes
        No data available
        Mapping
        No data available
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        Citation
        Chng, S.C., Ho, L., Tian, J., and Reversade, B. (2013) ELABELA: A Hormone Essential for Heart Development Signals via the Apelin Receptor. Developmental Cell. 27(6):672-680.