PUBLICATION

A BMP regulatory network controls ectodermal cell fate decisions at the neural plate border

Authors
Reichert, S., Randall, R.A., and Hill C.S.
ID
ZDB-PUB-131107-4
Date
2013
Source
Development (Cambridge, England)   140(21): 4435-4444 (Journal)
Registered Authors
Randall, Becky, Reichert, Sabine
Keywords
BMP signalling, Bambib, Bmper, Neural crest, Neural plate border, Preplacodal ectoderm, Zebrafish
MeSH Terms
  • Signal Transduction/physiology*
  • Neural Crest/embryology
  • Neural Plate/embryology
  • Animals, Genetically Modified
  • Humans
  • Ectoderm/physiology*
  • Real-Time Polymerase Chain Reaction
  • Animals
  • Cell Differentiation/physiology*
  • Zebrafish Proteins/metabolism*
  • GTPase-Activating Proteins/metabolism*
  • Zebrafish/embryology*
  • Bone Morphogenetic Proteins/metabolism*
  • Blotting, Western
  • Alcian Blue
  • Neurulation/physiology*
  • In Situ Hybridization, Fluorescence
  • Immunoprecipitation
  • HEK293 Cells
(all 19)
PubMed
24089471 Full text @ Development
Abstract

During ectodermal patterning the neural crest and preplacodal ectoderm are specified in adjacent domains at the neural plate border. BMP signalling is required for specification of both tissues, but how it is spatially and temporally regulated to achieve this is not understood. Here, using a transgenic zebrafish BMP reporter line in conjunction with double-fluorescent in situ hybridisation, we show that, at the beginning of neurulation, the ventral-to-dorsal gradient of BMP activity evolves into two distinct domains at the neural plate border: one coinciding with the neural crest and the other abutting the epidermis. In between is a region devoid of BMP activity, which is specified as the preplacodal ectoderm. We identify the ligands required for these domains of BMP activity. We show that the BMP-interacting protein Crossveinless 2 is expressed in the BMP activity domains and is under the control of BMP signalling. We establish that Crossveinless 2 functions at this time in a positive-feedback loop to locally enhance BMP activity, and show that it is required for neural crest fate. We further demonstrate that the Distal-less transcription factors Dlx3b and Dlx4b, which are expressed in the preplacodal ectoderm, are required for the expression of a cell-autonomous BMP inhibitor, Bambi-b, which can explain the specific absence of BMP activity in the preplacodal ectoderm. Taken together, our data define a BMP regulatory network that controls cell fate decisions at the neural plate border.

Genes / Markers
Figures
Figure Gallery (10 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
cj100TgTransgenic Insertion
    w30TgTransgenic Insertion
      1 - 2 of 2
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      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      Target Reagent Reagent Type
      bambibMO1-bambibMRPHLNO
      bmp6MO1-bmp6MRPHLNO
      bmperMO1-bmperMRPHLNO
      bmperMO2-bmperMRPHLNO
      bmperMO4-bmperMRPHLNO
      dlx3bMO5-dlx3bMRPHLNO
      dlx4bMO5-dlx4bMRPHLNO
      gdf6aMO1-gdf6aMRPHLNO
      gdf6aMO5-gdf6aMRPHLNO
      tp53MO4-tp53MRPHLNO
      1 - 10 of 10
      Show
      Fish
      Antibodies
      Name Type Antigen Genes Isotypes Host Organism
      Ab1-smadpolyclonalIgGRabbit
      Ab1-smad5polyclonalIgGRabbit
      1 - 2 of 2
      Show
      Orthology
      No data available
      Engineered Foreign Genes
      Marker Marker Type Name
      GFPEFGGFP
      mRFPEFGmRFP
      1 - 2 of 2
      Show
      Mapping
      No data available