PUBLICATION

Wnt-Dependent Epithelial Transitions Drive Pharyngeal Pouch Formation

Authors
Choe, C.P., Collazo, A., Trinh, L.A., Pan, L., Moens, C.B., and Crump, J.G.
ID
ZDB-PUB-130213-8
Date
2013
Source
Developmental Cell   24(3): 296-309 (Journal)
Registered Authors
Crump, Gage DeKoeyer, Moens, Cecilia, Pan, Luyuan, Trinh, Le
Keywords
none
MeSH Terms
  • Pharynx/growth & development
  • Pharynx/metabolism
  • Adherens Junctions/metabolism
  • Wnt Proteins*/genetics
  • Wnt Proteins*/metabolism
  • Body Patterning*/genetics
  • Body Patterning*/physiology
  • Epithelium/growth & development
  • Mesoderm/growth & development
  • Mesoderm/metabolism
  • Wnt4 Protein*/genetics
  • Wnt4 Protein*/metabolism
  • Signal Transduction
  • Wnt Signaling Pathway
  • Zebrafish Proteins*/genetics
  • Zebrafish Proteins*/metabolism
  • Zebrafish*/genetics
  • Zebrafish*/growth & development
  • Embryonic Development
  • Animals
  • rac1 GTP-Binding Protein/genetics
  • rac1 GTP-Binding Protein/metabolism
  • Gene Expression Regulation, Developmental
(all 23)
PubMed
23375584 Full text @ Dev. Cell
Abstract

The pharyngeal pouches, which form by budding of the foregut endoderm, are essential for segmentation of the vertebrate face. To date, the cellular mechanism and segmental nature of such budding have remained elusive. Here, we find that Wnt11r and Wnt4a from the head mesoderm and ectoderm, respectively, play distinct roles in the segmental formation of pouches in zebrafish. Time-lapse microscopy, combined with mutant and tissue-specific transgenic experiments, reveal requirements of Wnt signaling in two phases of endodermal epithelial transitions. Initially, Wnt11r and Rac1 destabilize the endodermal epithelium to promote the lateral movement of pouch-forming cells. Next, Wnt4a and Cdc42 signaling induce the rearrangement of maturing pouch cells into bilayers through junctional localization of the Alcama immunoglobulin-domain protein, which functions to restabilize adherens junctions. We propose that this dynamic control of epithelial morphology by Wnt signaling may be a common theme for the budding of organ anlagen from the endoderm.

Genes / Markers
Figures
Figure Gallery (9 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
ct3aGtTransgenic Insertion
el48TgTransgenic Insertion
    el53TgTransgenic Insertion
      el72TgTransgenic Insertion
        el74TgTransgenic Insertion
          el83TgTransgenic Insertion
            el93TgTransgenic Insertion
              el190TgTransgenic Insertion
                el262TgTransgenic Insertion
                  el268TgTransgenic Insertion
                    1 - 10 of 17
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                    Human Disease / Model
                    No data available
                    Sequence Targeting Reagents
                    Target Reagent Reagent Type
                    alcamaMO1-alcamaMRPHLNO
                    fzd8aMO2-fzd8aMRPHLNO
                    wnt4MO4-wnt4MRPHLNO
                    1 - 3 of 3
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                    Fish
                    Antibodies
                    Orthology
                    No data available
                    Engineered Foreign Genes
                    Marker Marker Type Name
                    CeruleanEFGCerulean
                    CFPEFGCFP
                    CitrineEFGCitrine
                    EGFPEFGEGFP
                    mCherryEFGmCherry
                    1 - 5 of 5
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                    Mapping
                    No data available