PUBLICATION

csrnp1a Is Necessary for the Development of Primitive Hematopoiesis Progenitors in Zebrafish

Authors
Espina, J., Feijoo, C.G., Solis, C., and Glavic, A.
ID
ZDB-PUB-130125-16
Date
2013
Source
PLoS One   8(1): e53858 (Journal)
Registered Authors
Espina, Jaime, Feijoo, Carmen G.
Keywords
none
MeSH Terms
  • Nuclear Proteins/genetics*
  • Nuclear Proteins/metabolism
  • Animals
  • Blood Cell Count
  • Cell Lineage
  • Hematopoiesis/genetics*
  • Zebrafish/genetics
  • Zebrafish/growth & development*
  • Gene Expression Regulation, Developmental
  • Head*/anatomy & histology
  • Head*/growth & development
  • Head*/pathology
  • Embryo, Nonmammalian/metabolism
  • Embryo, Nonmammalian/physiopathology
  • Animals, Genetically Modified
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
  • Larva/genetics
  • Larva/growth & development
(all 19)
PubMed
23326522 Full text @ PLoS One
Abstract

The CSRNP (cystein-serine-rich nuclear protein) transcription factors are conserved from Drosophila to human. Functional studies in mice, through knockout for each of their paralogs, have resulted insufficient to elucidate the function of this family of proteins in vertebrate development. Previously, we described the function of the zebrafish ortholog, Csnrp1/Axud1, showing its essential role in the survival and proliferation of cephalic progenitors. To extend our understanding of this family, we have studied the function of its paralog csrnp1a. Our results show that csrnp1a is expressed from 0 hpf, until larval stages, particularly in cephalic territories and in the intermediate cell mass (ICM). Using morpholinos in wild type and transgenic lines we observed that Csrnp1a knockdown generates a mild reduction in head size and a depletion of blood cells in circulation. This was combined with in situ hybridizations to analyze the expression of different mesodermal and primitive hematopoiesis markers. Morphant embryos have impaired blood formation without disruption of mesoderm specification, angiogenesis or heart development. The reduction of circulating blood cells occurs at the hematopoietic progenitor level, affecting both the erythroid and myeloid lineages. In addition, cell proliferation was also altered in hematopoietic anterior sites, specifically in spi1 expression domain. These and previous observations suggest an important role of Csnrps transcription factors in progenitor biology, both in the neural and hematopoietic linages.

Genes / Markers
Figures
Figure Gallery (9 images)
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
i114TgTransgenic Insertion
    y1TgTransgenic Insertion
      1 - 2 of 2
      Show
      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      Target Reagent Reagent Type
      csrnp1aMO1-csrnp1aMRPHLNO
      csrnp1aMO2-csrnp1aMRPHLNO
      csrnp1bMO1-csrnp1bMRPHLNO
      tp53MO4-tp53MRPHLNO
      1 - 4 of 4
      Show
      Fish
      Antibodies
      No data available
      Orthology
      No data available
      Engineered Foreign Genes
      Marker Marker Type Name
      EGFPEFGEGFP
      GFPEFGGFP
      1 - 2 of 2
      Show
      Mapping
      No data available