PUBLICATION

Fgf signaling governs cell fate in the zebrafish pineal complex

Authors
Clanton, J.A., Hope, K.D., and Gamse, J.T.
ID
ZDB-PUB-121221-2
Date
2013
Source
Development (Cambridge, England)   140(2): 323-332 (Journal)
Registered Authors
Clanton, Joshua, Gamse, Josh
Keywords
specification, determination, differentiation, epithalamus, zebrafish, neurons
MeSH Terms
  • Zebrafish
  • Zebrafish Proteins/metabolism*
  • Fibroblast Growth Factors/metabolism*
  • In Situ Hybridization
  • Brain/embryology*
  • Brain/metabolism*
  • Cell Differentiation
  • Neurons/metabolism*
  • Enzyme Inhibitors/pharmacology
  • Heat-Shock Proteins/metabolism
  • Epithalamus/metabolism
  • Gene Expression Regulation, Developmental*
  • Signal Transduction
  • Cell Lineage
  • Pineal Gland/embryology*
  • Animals
  • Microscopy, Fluorescence/methods
  • Mutation
(all 18)
PubMed
23250206 Full text @ Development
Abstract

Left-right (L-R) asymmetries in neuroanatomy exist throughout the animal kingdom, with implications for function and behavior. The molecular mechanisms that control formation of such asymmetries are beginning to be understood. Significant progress has been made by studying the zebrafish parapineal organ, a group of neurons on the left side of the epithalamus. Parapineal cells arise from the medially located pineal complex anlage and migrate to the left side of the brain. We have found that Fgf8a regulates a fate decision among anterior pineal complex progenitors that occurs just prior to the initiation of leftward migration. Cell fate analysis shows that in the absence of Fgf8a a subset of cells in the anterior pineal complex anlage differentiate as cone photoreceptors rather than parapineal neurons. Fgf8a acts permissively to promote parapineal fate in conjunction with the transcription factor Tbx2b, but might also block cone photoreceptor fate. We conclude that this subset of anterior pineal complex precursors, which normally become parapineal cells, are bipotential and require Fgf8a to maintain parapineal identity and/or prevent cone identity.

Genes / Markers
Figures
Figure Gallery (15 images) / 2
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b1193TgTransgenic Insertion
    c144
      Point Mutation
      vu376TgTransgenic Insertion
        x15
          Point Mutation
          zf104TgTransgenic Insertion
            1 - 5 of 5
            Show
            Human Disease / Model
            No data available
            Sequence Targeting Reagents
            Target Reagent Reagent Type
            notoMO3-notoMRPHLNO
            tbx2bMO4-tbx2bMRPHLNO
            1 - 2 of 2
            Show
            Fish
            Antibodies
            Name Type Antigen Genes Isotypes Host Organism
            Ab1-elavlmonoclonalIgG2bMouse
            zpr-1monoclonalIgG1Mouse
            1 - 2 of 2
            Show
            Orthology
            No data available
            Engineered Foreign Genes
            Marker Marker Type Name
            GFPEFGGFP
            KaedeEFGKaede
            1 - 2 of 2
            Show
            Mapping
            No data available