PUBLICATION

Morphogenesis and Cell Fate Determination within the Adaxial Cell Equivalence Group of the Zebrafish Myotome

Authors

Nguyen-Chi, M.E., Bryson-Richardson, R., Sonntag, C., Hall, T.E., Gibson, A., Sztal, T., Chua, W., Schilling, T.F., and Currie, P.D.

ID

ZDB-PUB-121121-36

Date

2012

Source

PLoS Genetics 8(10): e1003014 (Journal)

Registered Authors

Bryson-Richardson, Robert, Chua, Wendy, Currie, Peter D., Gibson, Abigail, Hall, Thomas, Schilling, Tom, Sonntag, Carmen, Sztal, Tamar Esther

Keywords

Embryos, BMP signaling, Somites, Hedgehog signaling, Muscle differentiation, Cell differentiation, Zebrafish, Precursor cells

MeSH Terms

Animals
Mutation
Base Sequence
Cell Differentiation*
Nerve Tissue Proteins/genetics
Nerve Tissue Proteins/metabolism
Bone Morphogenetic Proteins/metabolism
Growth Differentiation Factor 6/metabolism
Fibroblast Growth Factors/metabolism
Morphogenesis*/genetics
Muscle Fibers, Slow-Twitch/cytology*
Muscle Fibers, Slow-Twitch/metabolism*
Stem Cells/cytology
Stem Cells/metabolism
Zebrafish Proteins/genetics
Zebrafish Proteins/metabolism
Signal Transduction
Hedgehog Proteins/metabolism
Zebrafish/genetics*
Zebrafish/metabolism*
Gene Knockdown Techniques

(all 21)

PubMed

23133395 Full text @ PLoS Genet.

Abstract

One of the central questions of developmental biology is how cells of equivalent potential—an equivalence group—come to adopt specific cellular fates. In this study we have used a combination of live imaging, single cell lineage analyses, and perturbation of specific signaling pathways to dissect the specification of the adaxial cells of the zebrafish embryo. We show that the adaxial cells are myogenic precursors that form a cell fate equivalence group of approximately 20 cells that consequently give rise to two distinct sub-types of muscle fibers: the superficial slow muscle fibers (SSFs) and muscle pioneer cells (MPs), distinguished by specific gene expression and cell behaviors. Using a combination of live imaging, retrospective and indicative fate mapping, and genetic studies, we show that MP and SSF precursors segregate at the beginning of segmentation and that they arise from distinct regions along the anterior-posterior (AP) and dorsal-ventral (DV) axes of the adaxial cell compartment. FGF signaling restricts MP cell fate in the anterior-most adaxial cells in each somite, while BMP signaling restricts this fate to the middle of the DV axis. Thus our results reveal that the synergistic actions of HH, FGF, and BMP signaling independently create a three-dimensional (3D) signaling milieu that coordinates cell fate within the adaxial cell equivalence group.

Genes / Markers

Figures

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Expression

Gene	Fish	Conditions	Stage	Qualifier	Anatomy	Assay	Figure
EGFP	pd1Tg	heat shock	10-13 somites to 14-19 somites		whole organism	IHC	Fig. 4
EGFP	pd1Tg	standard conditions	10-13 somites to 14-19 somites	Not Detected	whole organism	IHC	Fig. 4
en2a	pd1Tg	heat shock	Prim-5		muscle pioneer	ISH	Fig. 4
en2a	pd1Tg	standard conditions	Prim-5		muscle pioneer	ISH	Fig. 4
en2a	WT	chemical treatment: SU5402	10-13 somites		adaxial cell	ISH	Fig. 4
en2a	WT	standard conditions	10-13 somites		adaxial cell	ISH	Fig. 1
en2a	WT	standard conditions	10-13 somites		adaxial cell	ISH	Fig. 4
en2a	WT	standard conditions	10-13 somites		muscle pioneer	ISH	Fig. 1
en2a	WT	standard conditions	10-13 somites		muscle pioneer	ISH	Fig. 4
en2a	WT	chemical treatment: SU5402	10-13 somites		muscle pioneer	ISH	Fig. 4

1 - 10 of 40

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
WT	chemical treatment: pharmaceutical	Prim-5	muscle pioneer increased amount, abnormal	Fig. 7
WT	chemical treatment: SU5402	Prim-5	migratory slow muscle precursor cell decreased amount, abnormal	Fig. 4
WT	chemical treatment: SU5402	Prim-5	muscle pioneer increased amount, abnormal	Fig. 5
WT	chemical treatment: SU5402	Prim-5	muscle pioneer increased amount, abnormal	Fig. 4
WT + MO1-gdf6a	chemical treatment: SU5402	Prim-5	muscle pioneer increased amount, abnormal	Fig. 7
WT + MO1-gdf6a	standard conditions	Prim-5	muscle pioneer increased amount, abnormal	Fig. 7
WT + MO1-gdf6a + MO4-tp53	standard conditions	Prim-5	muscle pioneer increased amount, abnormal	Fig. 7
gdf6a^s327/s327	standard conditions	Prim-5	muscle pioneer increased amount, abnormal	Fig. 7
pd1Tg	heat shock	Prim-5	migratory slow muscle precursor cell malformed, abnormal	Fig. 4
pd1Tg	heat shock	Prim-5	muscle pioneer increased amount, abnormal	Fig. 4

1 - 10 of 12

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
fh117		Point Mutation	spry4
ir1189Tg	Tg(5xBMPRE-Xla.Id3:GFP)	Transgenic Insertion
pd1Tg	Tg(hsp70l:dnfgfr1a-EGFP)	Transgenic Insertion
s327		Point Mutation	gdf6a

1 - 4 of 4

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Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
gdf6a	MO1-gdf6a	MRPHLNO
tp53	MO4-tp53	MRPHLNO

Fish

Fish
gdf6a^s327/s327
ir1189Tg
ir1189Tg + MO1-gdf6a
pd1Tg
spry4^fh117/fh117
spry4^fh117/fh117
WT
WT + MO1-gdf6a
WT + MO1-gdf6a + MO4-tp53

Antibodies

Name	Type	Antigen Genes	Isotypes	Host Organism
Ab1-eng	monoclonal	en1a en1b en2a en2b	IgG1	Mouse
Ab-F59	monoclonal		IgG1	Mouse

Orthology

Engineered Foreign Genes

Marker	Marker Type	Name
EGFP	EFG	EGFP
GFP	EFG	GFP

Mapping

Nguyen-Chi et al., 2012 ZDB-PUB-121121-36 PMID:23133395

Morphogenesis and Cell Fate Determination within the Adaxial Cell Equivalence Group of the Zebrafish Myotome

Nguyen-Chi et al., 2012

ZDB-PUB-121121-36
PMID:23133395