PUBLICATION

Subnuclear development of the zebrafish habenular nuclei requires ER translocon function

Authors
Doll, C.A., Burkart, J.T., Hope, K.D., Halpern, M.E., and Gamse, J.T.
ID
ZDB-PUB-111011-18
Date
2011
Source
Developmental Biology   360(1): 44-57 (Journal)
Registered Authors
Doll, Caleb, Gamse, Josh, Halpern, Marnie E.
Keywords
neurogenesis, left-right assymetry, epithalamus, endoplasmic reticulum
MeSH Terms
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/physiology
  • Habenula/cytology
  • Habenula/embryology*
  • Habenula/physiology
  • Base Sequence
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/physiology*
  • Animals
  • Models, Neurological
  • Phenotype
  • Nodal Signaling Ligands/genetics
  • Nodal Signaling Ligands/physiology
  • DNA Primers/genetics
  • Endoplasmic Reticulum/physiology
  • Mutation
  • Body Patterning/genetics
  • Body Patterning/physiology
  • Neurons/cytology
  • Membrane Proteins/genetics*
  • Membrane Proteins/physiology*
  • Neurogenesis/genetics
  • Neurogenesis/physiology
(all 24)
PubMed
21945073 Full text @ Dev. Biol.
Abstract

The dorsal habenular nuclei (Dh) of the zebrafish are characterized by significant left–right differences in gene expression, anatomy, and connectivity. Notably, the lateral subnucleus of the Dh (LsDh) is larger on the left side of the brain than on the right, while the medial subnucleus (MsDh) is larger on the right compared to the left. A screen for mutations that affect habenular laterality led to the identification of the sec61a-like 1(sec61al1) gene. In sec61al1c163 mutants, more neurons in the LsDh and fewer in the MsDh develop on both sides of the brain. Generation of neurons in the LsDh occurs more rapidly and continues for a longer time period in mutants than in WT. Expression of Nodal pathway genes on the left side of the embryos is unaffected in mutants, as is the left sided placement of the parapineal organ, which promotes neurogenesis in the LsDh of WT embryos. Ultrastructural analysis of the epithalamus indicates that ventricular precursor cells, which form an epithelium in WT embryos, lose apical-basal polarity in sec61al1c163 mutants. Our results show that in the absence of sec61al1, an excess of precursor cells for the LsDh exit the ventricular region and differentiate, resulting in formation of bilaterally symmetric habenular nuclei.

Genes / Markers
Figures
Figure Gallery (11 images) / 2
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
c163
    Point Mutation
    hi1058TgTransgenic Insertion
    1 - 2 of 2
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    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    Target Reagent Reagent Type
    sec61a1aMO1-sec61a1aMRPHLNO
    sec61a1aMO2-sec61a1aMRPHLNO
    tbx2bMO1-tbx2bMRPHLNO
    1 - 3 of 3
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    Fish
    Antibodies
    Orthology
    No data available
    Engineered Foreign Genes
    No data available
    Mapping
    Entity Type Entity Symbol Location
    Featurec163Chr: 6 Details
    GENEsec61a1aChr: 6 Details
    SSLPz7248Chr: 6 Details
    SSLPz22745Chr: 6 Details
    1 - 4 of 4
    Show