PUBLICATION

Meis1 specifies positional information in the retina and tectum to organize the zebrafish visual system

Authors
Erickson, T., French, C.R., and Waskiewicz, A.J.
ID
ZDB-PUB-100910-18
Date
2010
Source
Neural Development   5: 22 (Journal)
Registered Authors
Waskiewicz, Andrew
Keywords
none
MeSH Terms
  • Carbocyanines
  • Retinal Ganglion Cells/physiology
  • Gene Expression Regulation, Developmental/genetics*
  • Superior Colliculi/cytology
  • Superior Colliculi/drug effects
  • Superior Colliculi/embryology*
  • Animals
  • Neoplasm Proteins/chemistry
  • Neoplasm Proteins/genetics*
  • Neoplasm Proteins/metabolism*
  • Signal Transduction/genetics
  • Homeodomain Proteins/chemistry
  • Homeodomain Proteins/genetics*
  • Homeodomain Proteins/metabolism*
  • Embryo, Nonmammalian
  • Zebrafish
  • Gene Expression Profiling/methods
  • Animals, Genetically Modified
  • Apolipoprotein A-II/genetics
  • Apolipoprotein A-II/metabolism
  • Growth Differentiation Factor 6/chemistry
  • Growth Differentiation Factor 6/genetics
  • Smad5 Protein/chemistry
  • Smad5 Protein/genetics
  • Zebrafish Proteins/chemistry
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Mutation/genetics
  • RNA, Messenger/pharmacology
  • Body Patterning/genetics
  • Oligodeoxyribonucleotides, Antisense/pharmacology
  • Amino Acids
  • Retina/cytology
  • Retina/drug effects
  • Retina/embryology*
(all 35)
PubMed
20809932 Full text @ Neural Dev.
Abstract
BACKGROUND: During visual system development, multiple signalling pathways cooperate to specify axial polarity within the retina and optic tectum. This information is required for the topographic mapping of retinal ganglion cell axons on the tectum. Meis1 is a TALE-class homeodomain transcription factor known to specify anterior-posterior identity in the hindbrain, but its role in patterning the visual system patterning has not been investigated. RESULTS: meis1 is expressed in both the presumptive retina and tectum. An analysis of retinal patterning reveals that Meis1 is required to correctly specify both dorsal-ventral and nasal-temporal identity in the zebrafish retina. Meis1-knockdown results in a loss of smad1 expression and an upregulation in follistatin expression, thereby causing lower levels of Bmp signalling and a partial ventralization of the retina. Additionally, Meis1-deficient embryos exhibit ectopic Fgf signalling in the developing retina and a corresponding loss of temporal identity. Meis1 also positively regulates ephrin gene expression in the tectum. Consistent with these patterning phenotypes, a knockdown of Meis1 ultimately results in retinotectal mapping defects. CONCLUSIONS: In this work we describe a novel role for Meis1 in regulating Bmp signalling and in specifying temporal identity in the retina. By patterning both the retina and tectum, Meis1 plays an important role in establishing the retinotectal map and organizing the visual system.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
No data available
Human Disease / Model
No data available
Sequence Targeting Reagents
Target Reagent Reagent Type
gdf6aMO1-gdf6aMRPHLNO
gdf6aMO5-gdf6aMRPHLNO
meis1bMO1-meis1bMRPHLNO
meis1bMO3-meis1bMRPHLNO
smad5MO3-smad5MRPHLNO
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Fish
Antibodies
Orthology
No data available
Engineered Foreign Genes
No data available
Mapping
No data available