PUBLICATION

Arteries provide essential guidance cues for lymphatic endothelial cells in the zebrafish trunk

Authors

Bussmann, J., Bos, F.L., Urasaki, A., Kawakami, K., Duckers, H.J., and Schulte-Merker, S.

ID

ZDB-PUB-100719-29

Date

2010

Source

Development (Cambridge, England) 137(16): 2653-2657 (Journal)

Registered Authors

Bos, Frank, Bussmann, Jeroen, Kawakami, Koichi, Schulte-Merker, Stefan

Keywords

Lymphatic system, Cell migration, Endothelium, Pattern formation, Zebrafish

MeSH Terms

Animals, Genetically Modified
Animals
Arteries/cytology
Cell Movement
Zebrafish/embryology*
Endothelial Cells/cytology*
Body Patterning*

(all 7)

PubMed

20610484 Full text @ Development

Abstract

The endothelial cells of the vertebrate lymphatic system assemble into complex networks, but local cues that guide the migration of this distinct set of cells are currently unknown. As a model for lymphatic patterning, we have studied the simple vascular network of the zebrafish trunk consisting of three types of lymphatic vessels that develop in close connection with the blood vasculature. We have generated transgenic lines that allow us to distinguish between arterial, venous and lymphatic endothelial cells (LECs) within a single zebrafish embryo. We found that LECs migrate exclusively along arteries in a manner that suggests that arterial endothelial cells serve as the LEC migratory substrate. In the absence of intersegmental arteries, LEC migration in the trunk is blocked. Our data therefore demonstrate a crucial role for arteries in LEC guidance.

Genes / Markers

Figures

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Expression

Gene	Fish	Conditions	Stage	Anatomy	Assay	Figure
EGFP	hu5333Tg; s843Tg	standard conditions	Day 5	artery intersegmental vessel	IFL	Fig. 1
EGFP	hu5333Tg; s843Tg	standard conditions	Day 5	dorsal aorta	IFL	Fig. 1
EGFP	hu5333Tg; s843Tg	standard conditions	Day 5	intersegmental vein blood vessel endothelial cell	IFL	Fig. 1
EGFP	hu5333Tg; s843Tg	standard conditions	Day 5	posterior cardinal vein blood vessel endothelial cell	IFL	Fig. 1
EGFP	hu5333Tg; y1Tg	standard conditions	Protruding-mouth	artery intersegmental vessel	IFL	Fig. 3
EGFP	hu5333Tg; y1Tg	standard conditions	Protruding-mouth	lymphatic system endothelial cell	IFL	Fig. 3
EGFP	map3k22^{nksagff27cGt/+}; hu5333Tg; nkuasgfp1aTg	standard conditions	Day 5	dorsal longitudinal lymphatic vessel	IFL	Fig. 2
EGFP	map3k22^{nksagff27cGt/+}; hu5333Tg; nkuasgfp1aTg	standard conditions	Day 5	intersegmental lymph vessel lymph vessel endothelium	IFL	Fig. 2
EGFP	map3k22^{nksagff27cGt/+}; hu5333Tg; nkuasgfp1aTg	standard conditions	Day 4	intersegmental lymph vessel lymph vessel endothelium	IFL	Fig. 4
EGFP	map3k22^{nksagff27cGt/+}; hu5333Tg; nkuasgfp1aTg	standard conditions	Protruding-mouth	lymphatic system endothelial cell	IFL	Fig. 3

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
map3k22^{nksagff27cGt/+}; hu5333Tg; nkuasgfp1aTg + MO1-kdrl	standard conditions	Day 4	intersegmental artery malformed, abnormal	Fig. 4
map3k22^{nksagff27cGt/+}; hu5333Tg; nkuasgfp1aTg + MO1-kdrl	standard conditions	Day 4	intersegmental artery mislocalised, abnormal	Fig. 4
map3k22^{nksagff27cGt/+}; hu5333Tg; nkuasgfp1aTg + MO1-kdrl	standard conditions	Day 4	intersegmental lymph vessel malformed, abnormal	Fig. 4
map3k22^{nksagff27cGt/+}; hu5333Tg; nkuasgfp1aTg + MO1-kdrl	standard conditions	Day 4	intersegmental lymph vessel mislocalised, abnormal	Fig. 4

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
hu4453Tg	TgBAC(stab1:YFP)	Transgenic Insertion
hu4624Tg	TgBAC(flt1:Citrine)	Transgenic Insertion
hu5088		Point Mutation	kdrl
hu5333Tg	Tg(-0.8flt1:RFP)	Transgenic Insertion
nksagff27cGt	Gt(GAL4FF)	Transgenic Insertion	map3k22
nkuasgfp1aTg	Tg(5xUAS:EGFP)	Transgenic Insertion
s843Tg	Tg(kdrl:EGFP)	Transgenic Insertion
t26480		Point Mutation	plcg1
y1Tg	Tg(fli1:EGFP)	Transgenic Insertion

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Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
kdrl	MO1-kdrl	MRPHLNO

Fish

Fish
hu4453Tg
hu4624Tg
hu5333Tg
hu5333Tg; s843Tg
hu5333Tg; y1Tg
map3k22^{nksagff27cGt/+}; hu5333Tg; nkuasgfp1aTg
map3k22^{nksagff27cGt/+}; hu5333Tg; nkuasgfp1aTg + MO1-kdrl
map3k22^{nksagff27cGt/+}; nkuasgfp1aTg
plcg1^{t26480/t26480}
y1Tg

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Antibodies

Orthology

Engineered Foreign Genes

Marker	Marker Type	Name
EGFP	EFG	EGFP
RFP	EFG	RFP
YFP	EFG	YFP

Mapping

Bussmann et al., 2010 ZDB-PUB-100719-29 PMID:20610484

Arteries provide essential guidance cues for lymphatic endothelial cells in the zebrafish trunk

Bussmann et al., 2010

ZDB-PUB-100719-29
PMID:20610484