PUBLICATION

A role for chemokine signaling in neural crest cell migration and craniofacial development

Authors
Olesnicky Killian, E.C., Birkholz, D.A., and Artinger, K.B.
ID
ZDB-PUB-090714-2
Date
2009
Source
Developmental Biology   333(1): 161-172 (Journal)
Registered Authors
Artinger, Kristin Bruk
Keywords
chemokine, cxcr4, sdf1, cxcr7, neural crest, migration, neurocranium, endoderm
MeSH Terms
  • Chemokine CXCL12/metabolism*
  • Embryo, Nonmammalian
  • Signal Transduction
  • Body Patterning/physiology
  • Animals
  • Cell Movement/physiology
  • Endoderm/embryology
  • Endoderm/metabolism
  • Zebrafish
  • Receptors, CXCR/metabolism*
  • Cartilage/embryology
  • Cartilage/metabolism
  • Zebrafish Proteins/metabolism*
  • Cell Differentiation/physiology
  • Neural Crest/embryology
  • Neural Crest/physiology*
  • Skull/embryology*
  • Skull/metabolism
  • Receptors, CXCR4/metabolism*
  • Branchial Region/embryology
  • Branchial Region/metabolism
(all 21)
PubMed
19576198 Full text @ Dev. Biol.
Abstract
Neural crest cells (NCCs) are a unique population of multipotent cells that migrate along defined pathways throughout the embryo and give rise to many diverse cell types including pigment cells, craniofacial cartilage and the peripheral nervous system (PNS). Aberrant migration of NCCs results in a wide variety of congenital birth defects including craniofacial abnormalities. The chemokine Sdf1 and its receptors, Cxcr4 and Cxcr7, have been identified as key components in the regulation of cell migration in a variety of tissues. Here we describe a novel role for the zebrafish chemokine receptor Cxcr4a in the development and migration of cranial NCCs (CNCCs). We find that loss of Cxcr4a, but not Cxcr7b results in aberrant CNCC migration, defects in the neurocranium, as well as cranial ganglia dismorphogenesis. Moreover, overexpression of either Sdf1b or Cxcr4a causes aberrant CNCC migration and results in ectopic craniofacial cartilages. We propose a model in which Sdf1b signaling from the pharyngeal arch endoderm and optic stalk to Cxcr4a expressing CNCCs is important for both the proper condensation of the CNCCs into pharyngeal arches and the subsequent patterning and morphogenesis of the neural crest derived tissues.
Genes / Markers
Figures
Figure Gallery (16 images) / 2
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b195
    		Insertion
    ba2TgTransgenic Insertion
      n1
        		Small Deletion
        y1TgTransgenic Insertion
          1 - 4 of 4
          Show
          Human Disease / Model
          No data available
          Sequence Targeting Reagents
          Target Reagent Reagent Type
          ackr3bMO6-ackr3bMRPHLNO
          cxcl12bMO1-cxcl12bMRPHLNO
          cxcr4aMO2-cxcr4aMRPHLNO
          cxcr4aMO3-cxcr4aMRPHLNO
          1 - 4 of 4
          Show
          Fish
          Fish
          ba2Tg
          noton1
          tbxtab195/b195
          y1Tg
          1 - 4 of 4
          Show
          Antibodies
          Orthology
          No data available
          Engineered Foreign Genes
          Marker Marker Type Name
          EGFPEFGEGFP
          1 - 1 of 1
          Show
          Mapping
          No data available
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          Citation
          Olesnicky Killian, E.C., Birkholz, D.A., and Artinger, K.B. (2009) A role for chemokine signaling in neural crest cell migration and craniofacial development. Developmental Biology. 333(1):161-172.