PUBLICATION

Apical polarity protein PrkCi is necessary for maintenance of spinal cord precursors in zebrafish

Authors
Roberts, R.K., and Appel, B.
ID
ZDB-PUB-090526-13
Date
2009
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   238(7): 1638-1648 (Journal)
Registered Authors
Appel, Bruce, Roberts, Randy
Keywords
neural precursors, zebrafish, oligodendrocytes, fate specification
MeSH Terms
  • Cell Differentiation/genetics
  • Motor Neurons/physiology
  • Animals
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Spinal Cord/embryology
  • Spinal Cord/metabolism
  • Spinal Cord/physiology*
  • Stem Cells/metabolism
  • Stem Cells/physiology*
  • Adherens Junctions/genetics
  • Adherens Junctions/physiology
  • Animals, Genetically Modified
  • Cell Polarity/genetics*
  • Isoenzymes/genetics
  • Isoenzymes/physiology*
  • Protein Kinase C/genetics
  • Protein Kinase C/physiology*
  • Cell Proliferation
  • Neuroepithelial Cells/metabolism
  • Neuroepithelial Cells/physiology
  • Embryo, Nonmammalian
(all 22)
PubMed
19449304 Full text @ Dev. Dyn.
Abstract
During development, neural precursors divide to produce new precursors and cells that differentiate as neurons and glia. In Drosophila, apicobasal polarity and orientation of the mitotic spindle play important roles in specifying the progeny of neural precursors for different fates. We examined orientation of zebrafish spinal cord precursors using time-lapse imaging and tested the function of protein kinase C, iota (PrkCi), a member of the Par complex of proteins necessary for apicobasal polarity in the nervous system. We found that nearly all precursors divide within the plane of the neuroepithelium of wild-type embryos even when they must produce cells that have different fates. In the absence of PrkCi function, neural precursor divisions become oblique during late embryogenesis and excess oligodendrocytes form concomitant with loss of dividing cells. We conclude that PrkCi function and planar divisions are necessary for asymmetric, self-renewing division of spinal cord precursors.
Genes / Markers
Figures
Figure Gallery (6 images)
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
kca66TgTransgenic Insertion
    m567
      Point Mutation
      vu12TgTransgenic Insertion
        1 - 3 of 3
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        Human Disease / Model
        No data available
        Sequence Targeting Reagents
        No data available
        Fish
        1 - 3 of 3
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        Antibodies
        Orthology
        No data available
        Engineered Foreign Genes
        Marker Marker Type Name
        EGFPEFGEGFP
        GFPEFGGFP
        1 - 2 of 2
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        Mapping
        No data available