PUBLICATION

The characterization of a zebrafish mid-hindbrain mutant, mid-hindbrain gone (mgo)

Authors
Shima, T., Znosko, W., and Tsang, M.
ID
ZDB-PUB-090324-9
Date
2009
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   238(4): 899-907 (Journal)
Registered Authors
Shima, Takaki, Tsang, Michael, Znosko, Wade
Keywords
zebrafish, mutant, mid-hind brain boundary, pax2a
MeSH Terms
  • Fibroblast Growth Factors/genetics
  • Fibroblast Growth Factors/metabolism
  • Basic Helix-Loop-Helix Transcription Factors/genetics
  • Basic Helix-Loop-Helix Transcription Factors/metabolism
  • Mesencephalon/embryology*
  • Mesencephalon/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • Somites/embryology
  • Somites/metabolism
  • Animals, Genetically Modified
  • RNA, Messenger/genetics
  • Rhombencephalon/embryology*
  • Rhombencephalon/metabolism*
  • Gene Expression Regulation, Developmental
  • Embryo, Nonmammalian/embryology
  • Embryo, Nonmammalian/metabolism
  • Mutation/genetics
  • Phenotype
  • PAX2 Transcription Factor/genetics
  • PAX2 Transcription Factor/metabolism
  • Animals
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism*
(all 25)
PubMed
19301393 Full text @ Dev. Dyn.
Abstract
The vertebrate mid-hindbrain boundary (MHB) is a crucial morphological structure required for patterning and neural differentiation of the midbrain and anterior hindbrain. We isolated a novel zebrafish mutant, MHB gone (mgo), that exhibited a defective MHB. Expression of engrailed3 in the prospective MHB was absent at the 1-somite stage, suggesting that initiation of the isthmic organizer was disrupted in mgo mutants. Complementation test with mgo and noi, in which the pax2a gene is mutated, infer that the mgo mutant may represent a novel noi allele. However, pronephric, otic vesicle, and commissural axonal defects described in noi mutants were not associated with mgo mutants. Genetic mapping revealed that the mgo mutation is linked to the Pax2a locus, but no mutation was detected in pax2a exons or within intron-exon boundaries. Based on these findings, we propose that the mgo mutation genetically interacts with pax2a required for the initiation of MHB formation.
Genes / Markers
Figures
Figure Gallery (8 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
pt10TgTransgenic Insertion
    pt11
      Unknown
      tu29a
        Point Mutation
        1 - 3 of 3
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        Human Disease / Model
        No data available
        Sequence Targeting Reagents
        Target Reagent Reagent Type
        wnt8bMO3-wnt8bMRPHLNO
        1 - 1 of 1
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        Fish
        Antibodies
        Orthology
        No data available
        Engineered Foreign Genes
        Marker Marker Type Name
        EGFPEFGEGFP
        1 - 1 of 1
        Show
        Mapping
        Entity Type Entity Symbol Location
        GENEmgoChr: 13 Details
        GENEpax2aChr: 13 Details
        1 - 2 of 2
        Show