PUBLICATION

Formation of the asymmetric pineal complex in zebrafish requires two independently acting transcription factors

Authors

Snelson, C.D., Burkart, J.T., and Gamse, J.T.

ID

ZDB-PUB-080722-13

Date

2008

Source

Developmental Dynamics : an official publication of the American Association of Anatomists 237(12): 3538-3544 (Journal)

Registered Authors

Gamse, Josh, Snelson, Corey

Keywords

homeodomain, t-box, epiphysis, epithalamus, dorsal diencephalon

MeSH Terms

Animals
T-Box Domain Proteins/genetics
T-Box Domain Proteins/metabolism*
Homeodomain Proteins/genetics
Homeodomain Proteins/metabolism*
Gene Expression Regulation, Developmental
Zebrafish Proteins/genetics
Zebrafish Proteins/metabolism*
Body Patterning*
Pineal Gland/cytology
Pineal Gland/embryology*
Pineal Gland/metabolism*
Zebrafish/embryology*
Zebrafish/genetics
Zebrafish/metabolism*
Transcription Factors/genetics
Transcription Factors/metabolism*
Mutation/genetics
Phenotype
Cell Division

(all 20)

PubMed

18629869 Full text @ Dev. Dyn.

Abstract

The pineal complex of zebrafish consists of a pineal organ and a left-sided parapineal organ. Mutation of the floating head (flh) gene, which encodes a homeodomain protein, causes premature termination of pineal cell division without affecting specification or asymmetric placement of the parapineal. The from beyond (fby) mutation, a premature stop codon in the T-domain-containing protein Tbx2b, disrupts formation of the parapineal while leaving the pineal largely intact. However, flh is reported as being required for tbx2b transcription. To resolve the paradox that flhand tbx2b mutants have opposite phenotypes but have been placed in the same genetic pathway, we have examined transcriptional cross-regulation in single flh or fby mutants and genetic epistasis in double mutants. Careful analysis shows that flh is not required for tbx2b transcription and double mutants exhibit an additive phenotype. We conclude that Flh and Tbx2b regulate separate programs of pineal and parapineal development.

Genes / Markers

Figures

Show all Figures

Expression

Gene	Antibody	Fish	Conditions	Stage	Anatomy	Assay	Figure
gfi1ab		AB	standard conditions	Day 4	parapineal organ	ISH	Fig. 3
gfi1ab		noto^n1/n1	standard conditions	Day 4	parapineal organ	ISH	Fig. 3
gfi1ab		tbx2b^c144/c144	standard conditions	Day 4	pineal complex	ISH	Fig. 5
gfi1ab		tbx2b^c144/c144; noto^n1/n1	standard conditions	Day 4	pineal complex	ISH	Fig. 5
GFP		noto^n1/n1; zf104Tg	standard conditions	Prim-15 to High-pec	epiphysis	IFL	Fig. 2
GFP	ab3-gfp	noto^n1/n1; zf104Tg	standard conditions	Day 4	epiphysis	IHC	Fig. 3
GFP	ab3-gfp	noto^n1/n1; zf104Tg	standard conditions	Day 4	parapineal organ	IHC	Fig. 3
GFP	ab3-gfp	noto^n1/n1; zf104Tg	standard conditions	14-19 somites	pineal complex	IHC	Fig. 1
GFP	ab3-gfp	noto^n1/n1; zf104Tg	standard conditions	Prim-5	pineal complex	IHC	Fig. 1
GFP		noto^n1/n1; zf104Tg	standard conditions	Prim-15 to High-pec	pineal complex	IFL	Fig. 2

1 - 10 of 30

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
noto^n1/n1; zf104Tg	standard conditions	Day 4	epiphysis decreased size, abnormal	Fig. 3
noto^n1/n1; zf104Tg	standard conditions	Day 4	pineal gland development disrupted, abnormal	Fig. 3
tbx2b^c144/c144	standard conditions	Day 4	epithalamus development disrupted, abnormal	Fig. 5
tbx2b^c144/c144	standard conditions	Day 4	parapineal organ cell mislocalised, abnormal	Fig. 5
tbx2b^c144/c144; noto^n1/n1; zf104Tg	standard conditions	Day 4	epiphysis decreased size, abnormal	Fig. 5
tbx2b^c144/c144; noto^n1/n1; zf104Tg	standard conditions	Day 4	epithalamus development disrupted, abnormal	Fig. 5
tbx2b^c144/c144; noto^n1/n1; zf104Tg	standard conditions	Day 4	pineal gland development disrupted, abnormal	Fig. 5

1 - 7 of 7

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
c144		Point Mutation	tbx2b
n1		Small Deletion	noto
zf104Tg	Tg(foxd3:GFP)	Transgenic Insertion

Human Disease / Model

Sequence Targeting Reagents

Fish

Fish
AB
noto^n1/n1
noto^n1/n1; zf104Tg
tbx2b^c144/c144
tbx2b^c144/c144; noto^n1/n1
tbx2b^c144/c144; noto^n1/n1; zf104Tg
tbx2b^c144/c144; zf104Tg
zf104Tg

Antibodies

Orthology

Engineered Foreign Genes

Marker	Marker Type	Name
GFP	EFG	GFP

Mapping

Snelson et al., 2008 ZDB-PUB-080722-13 PMID:18629869

Formation of the asymmetric pineal complex in zebrafish requires two independently acting transcription factors

Snelson et al., 2008

ZDB-PUB-080722-13
PMID:18629869