PUBLICATION

Formation of the asymmetric pineal complex in zebrafish requires two independently acting transcription factors

Authors
Snelson, C.D., Burkart, J.T., and Gamse, J.T.
ID
ZDB-PUB-080722-13
Date
2008
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   237(12): 3538-3544 (Journal)
Registered Authors
Gamse, Josh, Snelson, Corey
Keywords
homeodomain, t-box, epiphysis, epithalamus, dorsal diencephalon
MeSH Terms
  • Animals
  • T-Box Domain Proteins/genetics
  • T-Box Domain Proteins/metabolism*
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/metabolism*
  • Gene Expression Regulation, Developmental
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • Body Patterning*
  • Pineal Gland/cytology
  • Pineal Gland/embryology*
  • Pineal Gland/metabolism*
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Transcription Factors/genetics
  • Transcription Factors/metabolism*
  • Mutation/genetics
  • Phenotype
  • Cell Division
(all 20)
PubMed
18629869 Full text @ Dev. Dyn.
Abstract
The pineal complex of zebrafish consists of a pineal organ and a left-sided parapineal organ. Mutation of the floating head (flh) gene, which encodes a homeodomain protein, causes premature termination of pineal cell division without affecting specification or asymmetric placement of the parapineal. The from beyond (fby) mutation, a premature stop codon in the T-domain-containing protein Tbx2b, disrupts formation of the parapineal while leaving the pineal largely intact. However, flh is reported as being required for tbx2b transcription. To resolve the paradox that flhand tbx2b mutants have opposite phenotypes but have been placed in the same genetic pathway, we have examined transcriptional cross-regulation in single flh or fby mutants and genetic epistasis in double mutants. Careful analysis shows that flh is not required for tbx2b transcription and double mutants exhibit an additive phenotype. We conclude that Flh and Tbx2b regulate separate programs of pineal and parapineal development.
Genes / Markers
Figures
Figure Gallery (5 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
c144
    Point Mutation
    n1
      Small Deletion
      zf104TgTransgenic Insertion
        1 - 3 of 3
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        Human Disease / Model
        No data available
        Sequence Targeting Reagents
        No data available
        Fish
        Antibodies
        Orthology
        No data available
        Engineered Foreign Genes
        Marker Marker Type Name
        GFPEFGGFP
        1 - 1 of 1
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        Mapping
        No data available