PUBLICATION

Intra-endodermal interactions are required for pancreatic beta cell induction

Authors
Chung, W.S., and Stainier, D.Y.
ID
ZDB-PUB-080421-3
Date
2008
Source
Developmental Cell   14(4): 582-593 (Journal)
Registered Authors
Chung, Won-Suk, Stainier, Didier
Keywords
DEVBIO
MeSH Terms
  • Mesoderm/cytology
  • Mesoderm/physiology
  • Insulin-Secreting Cells/cytology
  • Insulin-Secreting Cells/physiology*
  • Signal Transduction/physiology*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Receptors, G-Protein-Coupled/genetics
  • Receptors, G-Protein-Coupled/metabolism
  • Embryonic Induction/physiology*
  • Cell Lineage
  • Hedgehog Proteins/genetics
  • Hedgehog Proteins/metabolism
  • Recombinant Fusion Proteins/genetics
  • Recombinant Fusion Proteins/metabolism
  • Animals, Genetically Modified
  • Zebrafish*/anatomy & histology
  • Zebrafish*/embryology
  • Transgenes
  • Cell Transplantation
  • Cell Differentiation/physiology*
  • Animals
  • Endoderm/cytology
  • Endoderm/physiology*
(all 24)
PubMed
18410733 Full text @ Dev. Cell
Abstract
The cellular origin of signals that regulate pancreatic beta cell induction is not clearly defined. Here, we investigate the seeming paradox that Hedgehog/Smoothened signaling functions during gastrulation to promote pancreatic beta cell development in zebrafish, yet has an inhibitory role during later stages of pancreas development in amniotes. Our cell transplantation experiments reveal that in zebrafish, Smoothened function is not required in beta cell precursors. At early somitogenesis stages, when the zebrafish endoderm first forms a sheet, pancreatic beta cell precursors lie closest to the midline; however, the requirement for Smoothened lies in their lateral neighbors, which ultimately give rise to the exocrine pancreas and intestine. Thus, pancreatic beta cell induction requires Smoothened function cell-nonautonomously during gastrulation, to allow subsequent intra-endodermal interactions. These results clarify the function of Hedgehog signaling in pancreas development, identify an unexpected cellular source of factors that regulate beta cell specification, and uncover complex patterning and signaling interactions within the endoderm.
Genes / Markers
Figures
Figure Gallery (13 images) / 2
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
hi1640TgTransgenic Insertion
n1
    Small Deletion
    s854TgTransgenic Insertion
      s870TgTransgenic Insertion
        s903TgTransgenic Insertion
          zf5TgTransgenic Insertion
            1 - 6 of 6
            Show
            Human Disease / Model
            No data available
            Sequence Targeting Reagents
            Target Reagent Reagent Type
            sox32MO3-sox32MRPHLNO
            1 - 1 of 1
            Show
            Fish
            Antibodies
            No data available
            Orthology
            No data available
            Engineered Foreign Genes
            Marker Marker Type Name
            DsRedEFGDsRed
            GFPEFGGFP
            1 - 2 of 2
            Show
            Mapping
            No data available