PUBLICATION

Crucial role of zebrafish prox1 in hypothalamic catecholaminergic neurons development

Authors

Pistocchi, A., Gaudenzi, G., Carra, S., Bresciani, E., Del Giacco, L., and Cotelli, F.

ID

ZDB-PUB-080324-5

Date

2008

Source

BMC Developmental Biology 8: 27 (Journal)

Registered Authors

Cotelli, Franco, Del Giacco, Luca

Keywords

none

MeSH Terms

Cell Count
Genes, Homeobox
Tumor Suppressor Proteins/genetics*
Neurons/cytology*
Hypothalamus/cytology
Hypothalamus/embryology*
Embryo, Nonmammalian/embryology
In Situ Nick-End Labeling
Zebrafish Proteins/genetics*
In Situ Hybridization
Catecholamines/genetics*
Zebrafish/embryology*
Zebrafish/genetics
Immunohistochemistry
Reverse Transcriptase Polymerase Chain Reaction
Cell Differentiation
Homeodomain Proteins/genetics*
Animals
Gene Expression Regulation, Developmental

(all 19)

PubMed

18331627 Full text @ BMC Dev. Biol.

Abstract

BACKGROUND: Prox1, the vertebrate homolog of prospero in Drosophila melanogaster, is a divergent homeogene that regulates cell proliferation, fate determination and differentiation during vertebrate embryonic development. RESULTS: Here we report that, in zebrafish, prox1 is widely expressed in several districts of the Central Nervous System (CNS). Specifically, we evidenced prox1 expression in a group of neurons, already positive for otp1, located in the hypothalamus at the level of the posterior tuberculum (PT). Prox1 knock-down determines the severe loss of hypothalamic catecholaminergic (CA) neurons, identified by tyrosine hydroxylase (TH) expression, and the synergistic prox1/otp1 overexpression induces the appearance of hypothalamic supernumerary TH-positive neurons and ectopic TH-positive cells on the yolk epitelium. CONCLUSIONS: Our findings indicate that prox1 activity is crucial for the proper development of the otp1-positive hypothalamic neuronal precursors to their terminal CA phenotype.

Genes / Markers

Figures

Show all Figures

Expression

Gene	Fish	Conditions	Stage	Anatomy	Assay	Figure
neurog1	AB	control	Prim-5	central nervous system	ISH	Fig. S5
neurog1	AB	control	Prim-5	hypothalamus	ISH	Fig. S5
neurog1	AB + MO1-prox1a	standard conditions	Prim-5	central nervous system	ISH	Fig. S5
neurog1	AB + MO1-prox1a	standard conditions	Prim-5	hypothalamus	ISH	Fig. S5
otpb	AB	standard conditions	Prim-25	caudal tuberculum	ISH	Fig. 3
otpb	AB	control	Long-pec	caudal tuberculum	ISH	Fig. 3
otpb	AB	control	Long-pec	hypophysis	ISH	Fig. 3
otpb	AB	standard conditions	Prim-25	hypophysis	ISH	Fig. 3
otpb	AB	control	Long-pec	hypothalamus	ISH	Fig. 3
otpb	AB	standard conditions	Prim-25	hypothalamus	ISH	Fig. 3

1 - 10 of 78

Show

Phenotype

Fish	Conditions	Stage	Phenotype	Figure
AB + MO1-prox1a	standard conditions	Prim-5	hypothalamus neuron decreased amount, abnormal	Fig. S3
AB + MO1-prox1a	standard conditions	Prim-25	hypothalamus neuron decreased amount, abnormal	Fig. 2
AB + MO1-prox1a	standard conditions	Long-pec	hypothalamus neuron decreased amount, abnormal	Fig. 3
AB + MO1-prox1a	standard conditions	Long-pec	hypothalamus neuron decreased amount, abnormal	Fig. S3

1 - 4 of 4

Show

Mutations / Transgenics

Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
otpb	MO1-otpb	MRPHLNO
prox1a	MO1-prox1a	MRPHLNO

Fish

Fish
AB
AB + MO1-prox1a

Antibodies

Orthology

Engineered Foreign Genes

Mapping

Pistocchi et al., 2008 ZDB-PUB-080324-5 PMID:18331627

Crucial role of zebrafish prox1 in hypothalamic catecholaminergic neurons development

Pistocchi et al., 2008

ZDB-PUB-080324-5
PMID:18331627