PUBLICATION

A role for the inositol kinase Ipk1 in ciliary beating and length maintenance

Authors

Sarmah, B., Winfrey, V.P., Olson, G.E., Appel, B., and Wente, S.R.

ID

ZDB-PUB-071210-31

Date

2007

Source

Proceedings of the National Academy of Sciences of the United States of America 104(50): 19843-19848 (Journal)

Registered Authors

Appel, Bruce

Keywords

cilia, inositol hexakisphosphate, kidney development, left–right asymmetry, zebrafish

MeSH Terms

Gene Expression Regulation, Developmental
Protozoan Proteins/genetics
Protozoan Proteins/metabolism
Microtubules/metabolism
Zebrafish*/embryology
Zebrafish*/genetics
Zebrafish*/metabolism
Body Patterning
Cell Line
Animals
Zebrafish Proteins/genetics
Zebrafish Proteins/metabolism*
Cilia/enzymology*
Cilia/physiology*
Phosphotransferases (Alcohol Group Acceptor)/genetics
Phosphotransferases (Alcohol Group Acceptor)/metabolism*
Animals, Genetically Modified
Biological Transport
Humans
Centrosome/metabolism
Gene Expression Regulation, Enzymologic

(all 21)

PubMed

18056639 Full text @ Proc. Natl. Acad. Sci. USA

Abstract

Cilia project from cells as membranous extensions, with microtubule structural cores assembling from basal bodies by intraflagellar transport (IFT). Here, we report a ciliary role for the inositol 1,3,4,5,6-pentakisphosphate 2-kinase (Ipk1) that generates inositol hexakisphosphate. In zebrafish embryos, reducing Ipk1 levels inhibited ciliary beating in Kupffer's vesicle and decreased ciliary length in the spinal canal, pronephric ducts, and Kupffer's vesicle. Electron microscopy showed that ciliary axonemal structures were not grossly altered. However, coincident knockdown of Ipk1 and IFT88 or IFT57 had synergistic perturbations. With GFP-Ipk1 enriched in centrosomes and basal bodies, we propose that Ipk1 plays a previously uncharacterized role in ciliary function.

Genes / Markers

Figures

Show all Figures

Expression

Gene	Fish	Conditions	Stage	Anatomy	Assay	Figure
dlc	WT	control	5-9 somites	segmental plate	ISH	Fig. S6
dlc	WT	control	5-9 somites	somite	ISH	Fig. S6
dlc	WT + MO1-ippk	standard conditions	5-9 somites	segmental plate	ISH	Fig. S6
dlc	WT + MO1-ippk	standard conditions	5-9 somites	somite	ISH	Fig. S6
egr2b	WT	control	5-9 somites	presumptive rhombomere 3	ISH	Fig. S6
egr2b	WT	control	5-9 somites	presumptive rhombomere 5	ISH	Fig. S6
egr2b	WT + MO1-ippk	standard conditions	5-9 somites	presumptive rhombomere 3	ISH	Fig. S6
egr2b	WT + MO1-ippk	standard conditions	5-9 somites	presumptive rhombomere 5	ISH	Fig. S6
myod1	WT	control	5-9 somites	somite	ISH	Fig. S6
myod1	WT + MO1-ippk	standard conditions	5-9 somites	somite	ISH	Fig. S6

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
WT	chemical treatment: adrenaline	Day 5	pigment granule dispersal rate, normal	Fig. 3
WT	chemical treatment: (R)-adrenaline	Day 5	pigment granule aggregation in cell center decreased rate, abnormal	Fig. 3
WT + MO1-ift57	standard conditions	Prim-5	heart tube position, abnormal	Fig. S7
WT + MO1-ift57 + MO1-ippk	standard conditions	Prim-5	heart tube position, abnormal	Fig. S7
WT + MO1-ift57 + MO1-ippk	standard conditions	Prim-5	heart tube position, abnormal	Fig. 3
WT + MO1-ift88 + MO1-ippk	standard conditions	Prim-5	heart tube position, abnormal	Fig. S7
WT + MO1-ift88 + MO1-ippk	standard conditions	Prim-5	heart tube position, abnormal	Fig. 3
WT + MO1-ippk	standard conditions	Prim-5	heart tube position, abnormal	Fig. S7
WT + MO1-ippk	standard conditions	1-4 somites	Kupffer's vesicle axoneme structure, normal	Fig. 2
WT + MO1-ippk	standard conditions	5-9 somites	Kupffer's vesicle cilium decreased functionality, abnormal	Fig. 1

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
tz288		Point Mutation	ift88

Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
ift57	MO1-ift57	MRPHLNO
ift88	MO1-ift88	MRPHLNO
ippk	MO1-ippk	MRPHLNO

Fish

Fish
ift88^tz288/+ + MO1-ippk
WT
WT + MO1-ift57
WT + MO1-ift57 + MO1-ippk
WT + MO1-ift88 + MO1-ippk
WT + MO1-ippk

Antibodies

Orthology

Engineered Foreign Genes

Mapping

Sarmah et al., 2007 ZDB-PUB-071210-31 PMID:18056639

A role for the inositol kinase Ipk1 in ciliary beating and length maintenance

Sarmah et al., 2007

ZDB-PUB-071210-31
PMID:18056639