PUBLICATION

Amotl2 is essential for cell movements in zebrafish embryo and regulates c-Src translocation

Authors
Huang, H., Lu, F.I., Jia, S., Meng, S., Cao, Y., Wang, Y., Ma, W., Yin, K., Wen, Z., Peng, J., Thisse, C., Thisse, B., and Meng, A.
ID
ZDB-PUB-070303-6
Date
2007
Source
Development (Cambridge, England)   134(5): 979-988 (Journal)
Registered Authors
Cao, Ying, Huang, Huizhe, Jia, Shunji, Lu, Fu-I, Meng, Anming, Peng, Jinrong, Thisse, Bernard, Thisse, Christine, Wen, Zilong
Keywords
Zebrafish, Epiboly, Convergent extension, c-Src, Angiomotin-like2
MeSH Terms
  • Fibroblast Growth Factors/physiology
  • Membrane Proteins/genetics
  • Membrane Proteins/physiology*
  • Embryo, Nonmammalian/physiology
  • Zebrafish/embryology
  • Zebrafish/physiology*
  • Protein Transport
  • Endosomes/physiology
  • Cell Movement/physiology
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology*
  • Signal Transduction
  • Animals
  • src-Family Kinases/metabolism*
  • Molecular Sequence Data
  • Amino Acid Sequence
  • Actin Cytoskeleton/physiology
  • rhoB GTP-Binding Protein/metabolism
  • Vesicular Transport Proteins/metabolism
  • Pseudopodia/physiology
  • Actins/physiology
  • Phosphorylation
  • Body Patterning
(all 23)
PubMed
17293535 Full text @ Development
Abstract
Angiomotin (Amot), the founding member of the Motin family, is involved in angiogenesis by regulating endothelial cell motility, and is required for visceral endoderm movement in mice. However, little is known about biological functions of the other two members of the Motin family, Angiomotin-like1 (Amotl1) and Angiomotin-like2 (Amotl2). Here, we have identified zebrafish amotl2 as an Fgf-responsive gene. Zebrafish amotl2 is expressed maternally and in restricted cell types zygotically. Knockdown of amotl2 expression delays epiboly and impairs convergence and extension movement, and amotl2-deficient cells in mosaic embryos fail to migrate properly. This coincides with loss of membrane protrusions and disorder of F-actin. Amotl2 partially co-localizes with RhoB-or EEA1-positive endosomes and the non-receptor tyrosine kinase c-Src. We further demonstrate that Amotl2 interacts preferentially with and facilitates outward translocation of the phosphorylated c-Src, which may in turn regulate the membrane architecture. These data provide the first evidence that amotl2 is essential for cell movements in vertebrate embryos.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
No data available
Human Disease / Model
No data available
Sequence Targeting Reagents
Target Reagent Reagent Type
amotl2aMO1-amotl2aMRPHLNO
amotl2aMO2-amotl2aMRPHLNO
1 - 2 of 2
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Fish
1 - 2 of 2
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Antibodies
No data available
Orthology
Gene Orthology
amotl2a
1 - 1 of 1
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Engineered Foreign Genes
No data available
Mapping
No data available