PUBLICATION

miles-apart-Mediated regulation of cell-fibronectin interaction and myocardial migration in zebrafish

Authors
Matsui, T., Raya, A., Callol-Massot, C., Kawakami, Y., Oishi, I., Rodriguez-Esteban, C., and Izpisúa Belmonte, J.C.
ID
ZDB-PUB-070210-11
Date
2007
Source
Nature clinical practice. Cardiovascular medicine   4(1): S77-S82 (Journal)
Registered Authors
Izpisúa Belmonte, Juan Carlos, Kawakami, Yasuhiko, Matsui, Takaaki, Oishi, Isao, Raya, Angel, Rodriguez-Esteban, Concepcion
Keywords
none
MeSH Terms
  • Receptors, Lysosphingolipid/genetics*
  • Animals
  • Fibronectins/physiology*
  • Genotype
  • Zebrafish
  • Myocytes, Cardiac/physiology*
  • Cell Communication
  • Cell Movement
  • Mutation
  • Organogenesis/genetics
  • Organogenesis/physiology*
(all 11)
PubMed
17230219 Full text @ Nat. Clin. Pract. Cardiovasc. Med.
Abstract
The migration of myocardial precursor cells towards the embryonic midline underlies the formation of the heart tube and is a key process of heart organogenesis. The zebrafish mutation miles-apart (mil), which affects the gene encoding a sphingosine-1-phosphate receptor, is characterized by defective migration of myocardial precursor cells and results in the formation of two laterally positioned hearts, a condition known as cardia bifida. The mechanism that disrupts myocardial migration in mil mutants remains largely unclear. To investigate how mil regulates this process, here we analyze the interactions between mil and other mediators of myocardial migration. We show that mil function is associated with the other known cardia bifida locus, natter/fibronectin (nat/fn), which encodes fibronectin, a major component of the extracellular matrix, in the control of myocardial migration. By using a primary culture system of embryonic zebrafish cells, we also show that signaling from the sphingosine-1-phosphate receptor regulates cell-fibronectin interactions in zebrafish. In addition, localized inhibition and activation of cell-fibronectin interactions during the stages of myocardial migration reveal that the temporal regulation of cell-fibronectin interaction by mil is required for proper myocardial migration. Our study reveals novel functional links between sphingosine-1-phosphate receptor signaling and cell-fibronectin interaction in the control of myocardial migration during zebrafish heart organogenesis.
Genes / Markers
Figures
No images available
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Expression
No data available
Phenotype
Fish Conditions Stage Phenotype Figure
s1pr2te273/te273; twu34Tg/twu34TgcontrolLong-pec
s1pr2te273/te273; twu34Tg/twu34TgcontrolLong-pec
s1pr2te273/te273; twu34Tg/twu34TgcontrolLong-pec
s1pr2te273/te273; twu34Tg/twu34Tg + MO4-fn1astandard conditionsLong-pec
s1pr2te273/te273; twu34Tg/twu34Tg + MO4-fn1astandard conditionsLong-pec
s1pr2te273/te273; twu34Tg/twu34Tg + MO4-fn1astandard conditionsLong-pec
1 - 6 of 6
Show
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
te273
    		Point Mutation
    twu34TgTransgenic Insertion
      tz257
        		Point Mutation
        1 - 3 of 3
        Show
        Human Disease / Model
        No data available
        Sequence Targeting Reagents
        Target Reagent Reagent Type
        fn1aMO4-fn1aMRPHLNO
        s1pr2MO1-s1pr2MRPHLNO
        1 - 2 of 2
        Show
        Fish
        Antibodies
        Orthology
        Engineered Foreign Genes
        Marker Marker Type Name
        EGFPEFGEGFP
        1 - 1 of 1
        Show
        Mapping
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        Citation
        Matsui, T., Raya, A., Callol-Massot, C., Kawakami, Y., Oishi, I., Rodriguez-Esteban, C., and Izpisúa Belmonte, J.C. (2007) miles-apart-Mediated regulation of cell-fibronectin interaction and myocardial migration in zebrafish. Nature clinical practice. Cardiovascular medicine. 4(1):S77-S82.