PUBLICATION

Requirements for Endothelin type-A receptors and Endothelin-1 signaling in the facial ectoderm for the patterning of skeletogenic neural crest cells in zebrafish

Authors
Nair, S., Li, W., Cornell, R., and Schilling, T.F.
ID
ZDB-PUB-061229-1
Date
2007
Source
Development (Cambridge, England)   134(2): 335-345 (Journal)
Registered Authors
Cornell, Robert, Schilling, Tom
Keywords
Ednra, Craniofacial, Pharyngeal arch, Neural crest, Danio rerio, Zebrafish
MeSH Terms
  • Animals
  • Mutation
  • Receptor, Endothelin A/genetics*
  • Receptor, Endothelin A/metabolism
  • Base Sequence
  • Signal Transduction
  • Body Patterning
  • Phylogeny
  • Mice
  • Neural Crest/embryology
  • Neural Crest/metabolism
  • Endothelin-1/genetics*
  • Endothelin-1/metabolism
  • Molecular Sequence Data
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Zebrafish/metabolism
  • DNA, Complementary/genetics
  • Joints/embryology
  • Joints/metabolism
  • Amino Acid Sequence
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
  • Sequence Homology, Amino Acid
  • Models, Biological
  • Skull/embryology
  • Skull/metabolism
(all 27)
PubMed
17166927 Full text @ Development
Abstract
Genetic studies in mice and zebrafish have revealed conserved requirements for Endothelin 1 (Edn1) signaling in craniofacial development. Edn1 acts through its cognate type-A receptor (Ednra) to promote ventral skeletal fates and lower-jaw formation. Here, we describe the isolation and characterization of two zebrafish ednra genes - ednra1 and ednra2 - both of which are expressed in skeletal progenitors in the embryonic neural crest. We show that they play partially redundant roles in lower-jaw formation and development of the jaw joint. Knockdown of Ednra1 leads to fusions between upper- and lower-jaw cartilages, whereas the combined loss of Ednra1 and Ednra2 eliminates the lower jaw, similar to edn1(-/-) mutants. edn1 is expressed in pharyngeal arch ectoderm, mesoderm and endoderm. Tissue-mosaic studies indicate that, among these tissues, a crucial source of Edn1 is the surface ectoderm. This ectoderm also expresses ednrA1 in an edn1-dependent manner, suggesting that edn1 autoregulates its own expression. Collectively, our results indicate that Edn1 from the pharyngeal ectoderm signals through Ednra proteins to direct early dorsoventral patterning of the skeletogenic neural crest.
Genes / Markers
Figures
Figure Gallery (8 images)
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
WT + MO1-ednrabstandard conditionsDay 4
WT + MO1-ednrabstandard conditionsDay 4
WT + MO1-ednrabstandard conditionsDay 4
WT + MO1-ednrabstandard conditionsDay 4
WT + MO1-ednrabstandard conditionsDay 4
WT + MO1-ednrab + MO2-ednraastandard conditionsDay 4
WT + MO1-ednrab + MO2-ednraastandard conditionsDay 4
WT + MO1-ednrab + MO2-ednraastandard conditionsDay 4
edn1tf216b/tf216bstandard conditionsDay 4
edn1tf216b/tf216bstandard conditionsDay 4
1 - 10 of 11
Show
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
tf216b
    		Point Mutation
    1 - 1 of 1
    Show
    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    Target Reagent Reagent Type
    ednraaMO2-ednraaMRPHLNO
    ednrabMO1-ednrabMRPHLNO
    1 - 2 of 2
    Show
    Fish
    Fish
    edn1tf216b/tf216b
    WT
    WT + MO1-ednrab
    WT + MO1-ednrab + MO2-ednraa
    WT + MO2-ednraa
    1 - 5 of 5
    Show
    Antibodies
    No data available
    Orthology
    No data available
    Engineered Foreign Genes
    No data available
    Mapping
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    Citation
    Nair, S., Li, W., Cornell, R., and Schilling, T.F. (2007) Requirements for Endothelin type-A receptors and Endothelin-1 signaling in the facial ectoderm for the patterning of skeletogenic neural crest cells in zebrafish. Development (Cambridge, England). 134(2):335-345.