PUBLICATION

N-cadherin is required for the polarized cell behaviors that drive neurulation in the zebrafish

Authors
Hong, E., and Brewster, R.
ID
ZDB-PUB-060906-17
Date
2006
Source
Development (Cambridge, England)   133(19): 3895-3905 (Journal)
Registered Authors
Brewster, Rachel, Hong, Elim
Keywords
none
MeSH Terms
  • Animals
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Nervous System/cytology
  • Nervous System/embryology*
  • Mutation
  • Neural Tube Defects/genetics
  • Neuroepithelial Cells/cytology
  • Neuroepithelial Cells/physiology
  • Epithelium/metabolism
  • Epithelium/ultrastructure
  • Models, Biological
  • Cadherins/genetics
  • Cadherins/physiology*
  • Cell Polarity/genetics
  • Morphogenesis/genetics
(all 16)
PubMed
16943271 Full text @ Development
Abstract
Through the direct analysis of cell behaviors, we address the mechanisms underlying anterior neural tube morphogenesis in the zebrafish and the role of the cell adhesion molecule N-cadherin (N-cad) in this process. We demonstrate that although the mode of neurulation differs at the morphological level between amphibians and teleosts, the underlying cellular mechanisms are conserved. Contrary to previous reports, the zebrafish neural plate is a multi-layered structure, composed of deep and superficial cells that converge medially while undergoing radial intercalation, to form a single cell-layered neural tube. Time-lapse recording of individual cell behaviors reveals that cells are polarized along the mediolateral axis and exhibit protrusive activity. In N-cad mutants, both convergence and intercalation are blocked. Moreover, although N-cad-depleted cells are not defective in their ability to form protrusions, they are unable to maintain them stably. Taken together, these studies uncover key cellular mechanisms underlying neural tube morphogenesis in teleosts, and reveal a role for cadherins in promoting the polarized cell behaviors that underlie cellular rearrangements and shape the vertebrate embryo.
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Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
p79emcf
    Point Mutation
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    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    Target Reagent Reagent Type
    cdh2MO1-cdh2MRPHLNO
    1 - 1 of 1
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    Fish
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