PUBLICATION

beamter/deltaC and the role of Notch ligands in the zebrafish somite segmentation, hindbrain neurogenesis and hypochord differentiation

Authors
Julich, D., Hwee, Lim C., Round, J., Nicolaije, C., Schroeder, J., Davies, A., Geisler, R., Lewis, J., Jiang, Y.J., Holley, S.A., Tübingen 2000 Screen Consortium.
ID
ZDB-PUB-050831-9
Date
2005
Source
Developmental Biology   286(2): 391-404 (Journal)
Registered Authors
Geisler, Robert, Holley, Scott, Jiang, Yun-Jin, Jülich, Dörthe, Lewis, Julian, Lim, Chiaw Hwee
Keywords
Zebrafish, Somite, Notch, After eight, DeltaD, Beamter, DeltaC, Oscillator
MeSH Terms
  • Animals
  • RNA, Messenger/analysis
  • Gene Expression Regulation, Developmental
  • Alleles
  • Ligands
  • Intracellular Signaling Peptides and Proteins
  • Membrane Proteins/genetics*
  • Membrane Proteins/physiology
  • Zebrafish/embryology*
  • Biological Clocks/physiology
  • Mutation
  • Receptors, Notch/metabolism
  • Somites*
  • Rhombencephalon/cytology
  • Rhombencephalon/embryology*
  • Rhombencephalon/growth & development
(all 16)
PubMed
16125692 Full text @ Dev. Biol.
Abstract
The Tubingen large-scale zebrafish genetic screen completed in 1996 identified a set of five genes required for orderly somite segmentation. Four of them have been molecularly identified and three were found to code for components of the Notch pathway, which are required for the coordinated oscillation of gene expression, known as the segmentation clock, in the presomitic mesoderm (PSM). Here, we show that the final member of the group, beamter (bea), codes for the Notch ligand DeltaC, and we present and characterize two new alleles, including one allele encoding for a protein truncated in the 7th EGF repeat and an allele deleting only the DSL domain which was previously shown to be necessary for ligand function. Interestingly however, when we over-express any of the mutant deltaC mRNAs, we observe antimorphic effects on both hindbrain neurogenesis and hypochord formation. Expression of bea/deltaC oscillates in the PSM, and a triple fluorescent in situ analysis of its oscillation in relation to that of other oscillating genes in the PSM reveals differences in subcellular localization of the oscillating mRNAs in individual cells in different oscillation phases. Mutations in aei/deltaD and bea/deltaC differ in the way they disrupt the oscillating expression of her1 and deltaC. Furthermore, we find that the double mutants have significantly stronger defects in hypochord formation but not in somitogenesis or hindbrain neurogenesis, indicating genetically that the two delta's may function either semi-redundantly or distinctly, depending upon context.
Genes / Markers
Figures
Figure Gallery (5 images)
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Expression
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay Figure
col2a1adlcthf102/thf102standard conditionsPrim-15ISH
col2a1adlcthf102/thf102standard conditionsPrim-15ISH
col2a1adlctit446/tit446standard conditionsPrim-15ISH
col2a1adlctit446/tit446standard conditionsPrim-15ISH
col2a1adlctm98/tm98standard conditionsPrim-15ISH
col2a1adlctm98/tm98standard conditionsPrim-15ISH
col2a1adlcto202/to202standard conditionsPrim-15ISH
col2a1adlcto202/to202standard conditionsPrim-15ISH
col2a1adldtg249/tg249standard conditionsPrim-15ISH
col2a1adldtg249/tg249standard conditionsPrim-15ISH
1 - 10 of 42
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Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
tg249
    		Point Mutation
    thf102
      		Small Deletion
      tit446
        		Point Mutation
        tm98
          		Point Mutation
          to202
            		Point Mutation
            tr233
              		Point Mutation
              tw212b
                		Point Mutation
                1 - 7 of 7
                Show
                Human Disease / Model
                No data available
                Sequence Targeting Reagents
                No data available
                Fish
                Fish
                dlcthf102/thf102
                dlctit446/tit446
                dlctm98/tm98
                dlcto202/to202
                dlctw212b/tw212b
                dldtg249/tg249
                dldtr233/tr233
                WT
                1 - 8 of 8
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                Antibodies
                No data available
                Orthology
                No data available
                Engineered Foreign Genes
                No data available
                Mapping
                Entity Type Entity Symbol Location
                Featuretm98Chr: 15 Details
                SSLPz7871Chr: 15 Details
                SSLPz8991Chr: 15 Details
                1 - 3 of 3
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                Citation
                Julich, D., Hwee, Lim C., Round, J., Nicolaije, C., Schroeder, J., Davies, A., Geisler, R., Lewis, J., Jiang, Y.J., Holley, S.A., Tübingen 2000 Screen Consortium. (2005) beamter/deltaC and the role of Notch ligands in the zebrafish somite segmentation, hindbrain neurogenesis and hypochord differentiation. Developmental Biology. 286(2):391-404.