PUBLICATION

E-cadherin is required for gastrulation cell movements in zebrafish

Authors
Shimizu, T., Yabe, T., Muraoka, O., Yonemura, S., Aramaki, S., Hatta, K., Bae, Y.K., Nojima, H., and Hibi, M.
ID
ZDB-PUB-050523-7
Date
2005
Source
Mechanisms of Development   122(6): 747-763 (Journal)
Registered Authors
Bae, Young Ki, Hatta, Kohei, Hibi, Masahiko, Muraoka, Osamu, Nojima, Hideaki, Shimizu, Takashi, Yabe, Taijirou
Keywords
E-cadherin; Gastrulation; Cell movements; Epiboly; Convergence and extension; Cell migration; Cell adhesion; Zebrafish
MeSH Terms
  • Exons
  • Movement
  • Cell Movement
  • Indoles/pharmacology
  • In Situ Hybridization
  • Sequence Homology, Nucleic Acid
  • Animals
  • Gastrula/physiology*
  • Temperature
  • Zebrafish
  • Immunohistochemistry
  • Neurons/metabolism
  • Phenotype
  • Alleles
  • Mutation*
  • Gene Expression Regulation, Developmental*
  • Cadherins/genetics
  • Cadherins/metabolism
  • Cadherins/physiology*
  • Molecular Sequence Data
  • Zygote
  • Boron Compounds/pharmacology
  • Cell Adhesion
  • Base Sequence
  • Oligonucleotides/pharmacology
  • Microscopy, Electron
(all 26)
PubMed
15905076 Full text @ Mech. Dev.
Abstract
E-cadherin is a member of the classical cadherin family and is known to be involved in cell-cell adhesion and the adhesion-dependent morphogenesis of various tissues. We isolated a zebrafish mutant (cdh1(rk3)) that has a mutation in the e-cadherin/cdh1 gene. The mutation rk3 is a hypomorphic allele, and the homozygous mutant embryos displayed variable phenotypes in gastrulation and tissue morphogenesis. The most severely affected embryos displayed epiboly delay, decreased convergence and extension movements, and the dissociation of cells from the embryos, resulting in early embryonic lethality. The less severely affected embryos survived through the pharyngula stage and showed flattened anterior neural tissue, abnormal positioning and morphology of the hatching gland, scattered trigeminal ganglia, and aberrant axon bundles from the trigeminal ganglia. Maternal-zygotic cdh1(rk3) embryos displayed epiboly arrest during gastrulation, in which the enveloping layer (EVL) and the yolk syncytial layer but not the deep cells (DC) completed epiboly. A similar phenotype was observed in embryos that received antisense morpholino oligonucleotides (cdh1MO) against E-cadherin, and in zebrafish epiboly mutants. Complementation analysis with the zebrafish epiboly mutant weg suggested that cdh1(rk3) is allelic to half baked/weg. Immunohistochemistry with an anti-beta-catenin antibody and electron microscopy revealed that adhesion between the DCs and the EVL was mostly disrupted but the adhesion between DCs was relatively unaffected in the MZcdh1(rk3) mutant and cdh1 morphant embryos. These data suggest that E-cadherin-mediated cell adhesion between the DC and EVL plays a role in the epiboly movement in zebrafish.
Genes / Markers
Figures
Figure Gallery (8 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
rk3
    Point Mutation
    tx230
      Point Mutation
      1 - 2 of 2
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      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      Target Reagent Reagent Type
      cdh1MO5-cdh1MRPHLNO
      cdh1MO6-cdh1MRPHLNO
      1 - 2 of 2
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      Fish
      Antibodies
      Name Type Antigen Genes Isotypes Host Organism
      AB1-cdh1polyclonalRabbit
      1 - 1 of 1
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      Orthology
      No data available
      Engineered Foreign Genes
      No data available
      Mapping
      Entity Type Entity Symbol Location
      Featurerk3Chr: 7 Details
      SSLPz8156Chr: 7 Details
      1 - 2 of 2
      Show