PUBLICATION

nlz Gene family is required for hindbrain patterning in the zebrafish

Authors
Hoyle, J., Tang, Y.P., Wiellette, E.L., Wardle, F.C., and Sive, H.
ID
ZDB-PUB-040326-3
Date
2004
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   229(4): 835-846 (Journal)
Registered Authors
Sive, Hazel, Wardle, Fiona, Wiellette, Elizabeth
Keywords
none
MeSH Terms
  • Animals
  • In Situ Hybridization
  • Ephrin-B2/genetics
  • Ephrin-B2/metabolism
  • Amino Acid Sequence
  • Embryo, Nonmammalian/metabolism
  • Molecular Sequence Data
  • Zebrafish/embryology*
  • Gene Expression
  • Rhombencephalon/anatomy & histology
  • Rhombencephalon/embryology*
  • Rhombencephalon/metabolism
  • MafB Transcription Factor
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Zebrafish Proteins/physiology*
  • PAX2 Transcription Factor
  • DNA-Binding Proteins/genetics
  • DNA-Binding Proteins/metabolism
  • DNA-Binding Proteins/physiology*
  • Morphogenesis
  • Early Growth Response Protein 2
  • Phylogeny
  • Transcription Factors/genetics
  • Transcription Factors/metabolism
  • Body Patterning
  • Nerve Tissue Proteins/genetics
  • Nerve Tissue Proteins/metabolism
  • Repressor Proteins/genetics
  • Repressor Proteins/metabolism
  • Repressor Proteins/physiology*
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/metabolism
(all 33)
PubMed
15042707 Full text @ Dev. Dyn.
Abstract
This study describes the conserved nlz gene family whose members encode unusual zinc finger proteins. In the zebrafish neurectoderm, both nlz1 and the newly isolated nlz2 are expressed in the presumptive hindbrain and midbrain/hindbrain boundary, where expression of nlz1 is dependent on pax2a. In addition, nlz2 is uniquely expressed more anteriorly, in the presumptive midbrain and diencephalon. Overexpression of Nlz proteins during gastrula stages inhibits hindbrain development. In particular, ectopically expressed Nlz1 inhibits formation of future rhombomeres 2 and 3 (r2, r3), whereas neighboring r1 and r4 are not affected. Conversely, simultaneous reduction of Nlz1 and Nlz2 protein function by expression of antisense morpholino-modified oligomers leads to expansion of future r3 and r5, with associated loss of r4. These data indicate that one function of the nlz gene family is to specify or maintain r4 identity, and to limit r3 and r5 during hindbrain formation.
Genes / Markers
Figures
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Expression
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay Figure
egr2bABstandard conditions5-9 somitesISH
egr2bABstandard conditionsBudISH
egr2bABstandard conditions14-19 somitesISH
en2bABstandard conditions5-9 somitesISH
en2bpax2aunspecifiedstandard conditions5-9 somitesNot DetectedISH
hoxb1aABstandard conditions5-9 somitesISH
hoxb1aABstandard conditions14-19 somitesISH
hoxb1apax2aunspecifiedstandard conditions14-19 somitesISH
hoxb1apax2aunspecifiedstandard conditions5-9 somitesISH
otx2bABstandard conditions90%-epibolyISH
1 - 10 of 25
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Phenotype
No data available
Mutations / Transgenics
Human Disease / Model
No data available
Sequence Targeting Reagents
Fish
Fish
AB
pax2aunspecified
1 - 2 of 2
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Antibodies
No data available
Orthology
Engineered Foreign Genes
No data available
Mapping
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Citation
Hoyle, J., Tang, Y.P., Wiellette, E.L., Wardle, F.C., and Sive, H. (2004) nlz Gene family is required for hindbrain patterning in the zebrafish. Developmental Dynamics : an official publication of the American Association of Anatomists. 229(4):835-846.