PUBLICATION

nlz Gene family is required for hindbrain patterning in the zebrafish

Authors
Hoyle, J., Tang, Y.P., Wiellette, E.L., Wardle, F.C., and Sive, H.
ID
ZDB-PUB-040326-3
Date
2004
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   229(4): 835-846 (Journal)
Registered Authors
Sive, Hazel, Wardle, Fiona, Wiellette, Elizabeth
Keywords
none
MeSH Terms
  • Early Growth Response Protein 2
  • Ephrin-B2/genetics
  • Ephrin-B2/metabolism
  • PAX2 Transcription Factor
  • Repressor Proteins/genetics
  • Repressor Proteins/metabolism
  • Repressor Proteins/physiology*
  • MafB Transcription Factor
  • Morphogenesis
  • Amino Acid Sequence
  • DNA-Binding Proteins/genetics
  • DNA-Binding Proteins/metabolism
  • DNA-Binding Proteins/physiology*
  • Rhombencephalon/anatomy & histology
  • Rhombencephalon/embryology*
  • Rhombencephalon/metabolism
  • Zebrafish/embryology*
  • Gene Expression
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/metabolism
  • Body Patterning
  • Molecular Sequence Data
  • Transcription Factors/genetics
  • Transcription Factors/metabolism
  • Embryo, Nonmammalian/metabolism
  • Nerve Tissue Proteins/genetics
  • Nerve Tissue Proteins/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Zebrafish Proteins/physiology*
  • Animals
  • In Situ Hybridization
  • Phylogeny
(all 33)
PubMed
15042707 Full text @ Dev. Dyn.
Abstract
This study describes the conserved nlz gene family whose members encode unusual zinc finger proteins. In the zebrafish neurectoderm, both nlz1 and the newly isolated nlz2 are expressed in the presumptive hindbrain and midbrain/hindbrain boundary, where expression of nlz1 is dependent on pax2a. In addition, nlz2 is uniquely expressed more anteriorly, in the presumptive midbrain and diencephalon. Overexpression of Nlz proteins during gastrula stages inhibits hindbrain development. In particular, ectopically expressed Nlz1 inhibits formation of future rhombomeres 2 and 3 (r2, r3), whereas neighboring r1 and r4 are not affected. Conversely, simultaneous reduction of Nlz1 and Nlz2 protein function by expression of antisense morpholino-modified oligomers leads to expansion of future r3 and r5, with associated loss of r4. These data indicate that one function of the nlz gene family is to specify or maintain r4 identity, and to limit r3 and r5 during hindbrain formation.
Genes / Markers
Figures
No images available
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
pax2a_unspecified
    		Unspecified
    1 - 1 of 1
    Show
    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    Target Reagent Reagent Type
    znf503MO3-znf503MRPHLNO
    znf503MO4-znf503MRPHLNO
    znf703MO3-znf703MRPHLNO
    znf703MO4-znf703MRPHLNO
    1 - 4 of 4
    Show
    Fish
    Fish
    AB
    pax2aunspecified
    1 - 2 of 2
    Show
    Antibodies
    No data available
    Orthology
    Gene Orthology
    znf503
    znf703
    1 - 2 of 2
    Show
    Engineered Foreign Genes
    No data available
    Mapping
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    Citation
    Hoyle, J., Tang, Y.P., Wiellette, E.L., Wardle, F.C., and Sive, H. (2004) nlz Gene family is required for hindbrain patterning in the zebrafish. Developmental Dynamics : an official publication of the American Association of Anatomists. 229(4):835-846.