PUBLICATION

Fibronectin Regulates Epithelial Organization during Myocardial Migration in Zebrafish

Authors
Trinh, L.A., and Stainier, D.Y.
ID
ZDB-PUB-040322-7
Date
2004
Source
Developmental Cell   6(3): 371-382 (Journal)
Registered Authors
Stainier, Didier, Trinh, Le
Keywords
none
MeSH Terms
  • Zebrafish
  • Embryo, Nonmammalian
  • Somites/metabolism
  • Animals
  • Cytoskeletal Proteins/metabolism
  • Heart/embryology*
  • Body Patterning/genetics
  • Body Patterning/physiology
  • Luminescent Proteins/metabolism
  • Fibronectins/genetics
  • Fibronectins/physiology*
  • In Situ Hybridization/methods
  • Myocardium/cytology*
  • DNA Mutational Analysis
  • Genotype
  • Green Fluorescent Proteins
  • Microinjections/methods
  • Oligonucleotides, Antisense/metabolism
  • Epithelial Cells/physiology*
  • Zebrafish Proteins
  • Gene Expression Regulation, Developmental
  • Myoblasts/physiology*
  • Protein Kinase C/metabolism
  • Immunohistochemistry
  • Cell Movement/physiology*
  • Chromosome Mapping/methods
  • Trans-Activators/metabolism
  • beta Catenin
  • Myosin Light Chains/metabolism
  • Cell Adhesion/genetics
  • Cell Adhesion/physiology
(all 31)
PubMed
15030760 Full text @ Dev. Cell
Abstract
Several genes have been implicated in heart tube formation, yet we know little about underlying cellular mechanisms. We analyzed the cellular architecture of the migrating myocardial precursors, and find that they form coherent epithelia that mature as they move medially. Mutant analyses indicate that the cardia bifida locus natter (nat) is required for the integrity of the myocardial epithelia. We positionally cloned nat and show that it encodes Fibronectin. During myocardial migration, Fibronectin is deposited at the midline between the endoderm and endocardial precursors, and laterally around the myocardial precursors. Further analyses show that Fibronectin deposition at the midline is required for the timely migration of myocardial precursors, but dispensable for the migration process itself. In the complete absence of Fibronectin, adherens junctions between myocardial precursors do not form properly, suggesting that cell-substratum interactions are required for epithelial organization. These data suggest that myocardial migration is dependent on epithelial integrity.
Genes / Markers
Figures
No images available
Show all Figures
Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
npas4l_unspecified
    		Unspecified
    sox32_unspecified
      		Unspecified
      tl43c
        		Point Mutation
        twu34TgTransgenic Insertion
          1 - 4 of 4
          Show
          Human Disease / Model
          No data available
          Sequence Targeting Reagents
          Target Reagent Reagent Type
          fn1aMO1-fn1aMRPHLNO
          1 - 1 of 1
          Show
          Fish
          Fish
          fn1atl43c/tl43c
          npas4lunspecified
          sox32unspecified
          WT
          1 - 4 of 4
          Show
          Antibodies
          No data available
          Orthology
          Engineered Foreign Genes
          Marker Marker Type Name
          EGFPEFGEGFP
          1 - 1 of 1
          Show
          Mapping
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          Citation
          Trinh, L.A., and Stainier, D.Y. (2004) Fibronectin Regulates Epithelial Organization during Myocardial Migration in Zebrafish. Developmental Cell. 6(3):371-382.