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Publication: Flannery et al., 2026 - Impact of maternal compensation on developmental phenotypes in a zebrafish model of severe congenital muscular dystrophy
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Fig 4 Evaluation of neuromuscular junction integrity in MZKOs.

A: Maximum intensity projections of muscle stained with α-bungarotoxin (BTX) to label Acetylcholine receptors on the sarcolemma (left), anti-SV2 antibody to label motor neuron terminals (middle), and merged images to evaluate colocalization (right) (20X magnification, scale bars: 100 µm for main panel, 20 µm for insets). B–D: Quantifications of NMJs within the myotomes showing no significant differences in α-BTX intensity (B), α-BTX puncta density (C), or SV2 puncta density (D), but a significant reduction in colocalization (E) in MZKOs (**p = 0.004). B’–D’: Quantifications of neuromuscular junctions at the myotendinous junctions showing significantly reduced α-BTX intensity (B’), significantly increased α-BTX puncta density (C’), no change in SV2 puncta density (D’), and a significant reduction in colocalization (E’) (***p < 0.001).

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