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Fig. 3 The fndc1 nonsense allele (fndc1 sa23734) is associated with increased bone mineral density and total vertebral cross-sectional area in zebrafish. (A) MicroCT scan images demonstrating 3D projections of the vertebra of an individual fish. Vertebra in a lateral plane (top left) and axial plane (bottom left). Total vertebral cross-sectional area is quantified by determining the area of each axial section (right). MicroCT scans of zebrafish (n=5 for each genotype) revealed that fndc1 mutants have (B) increased vertebral bone mineral density (fndc1 +/+ vs fndc1 +/sa23734: p=0.034; fndc1 +/sa23734 vs fndc1 sa23734/sa23734: p=0.00019; fndc1 +/+ vs fndc1 sa23734/sa23734: p=6.08E-06) and (C) increased vertebral cross-sectional area compared with wild-type siblings (fndc1 +/+ vs fndc1 +/sa23734: p=0.0011; fndc1 +/sa23734 vs fndc1 sa23734/sa23734: p=0.94; fndc1 +/+ vs fndc1 sa23734/sa23734: p=1.37E-05). P values were calculated using the Wilcoxon rank-sum test, two sided. * indicates p< 0.05, ** for p < 0.005, *** for p<0.0005, and **** for p < 0.00005.