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Fig. 1 fzd5 is expressed in the optic primordium throughout eye formation. (A-D) Dorsal (A-C) or ventral (D) views, with anterior to the left, at the stage indicated in each panel. Dotted outlines in A and B highlight the optic vesicles. Brackets in D highlight the ciliary marginal zone (CMZ). (E) Schematic of the transcription activator-like effector nucleases (TALENs; t1 and t2) and guide RNAs (g1 to g4) used to generate the fzd5 mutant alleles for this study, and their predicted protein products. aa, amino acids; hpf, h post-fertilisation; hyp, hypothalamus; ov, optic vesicles; vt, ventral telencephalon. Scale bars: 100 µm.