ZFIN Image: Lee et al., 2025, Fig. 2.

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Figure Caption

Fig. 2.

Zebrafish slc25a22a is expressed in the brain, intestine and swim bladder, and Slc25a22a is localized in mitochondria of the forebrain. (A) Upper row: wild-type (WT) zebrafish larvae at 3-5 days post-fertilization (dpf) were probed with the slc25a22a riboprobe. Lateral view anterior to the left. The boxed areas are magnified in the respective panels in the lower row. Left, lateral view; right, dorsal view anterior to the left. Scale bars: 300 μm (upper); 100 μm (lower). (B) Cross-section images of the intestine and swim bladder of larvae at 5 dpf in A. Scale bar: 10 μm. (C) Co-transfection of COS-7 cells with the plasmid encoding GFP-tagged zebrafish Slc25a22a and the plasmid encoding mitochondrially targeted DsRed. Scale bar: 20 μm. (D) GFP-tagged slc25a22a RNAs were microinjected into a one-cell-stage Tg(mito:dsred) embryo, and its forebrain region was imaged at 1 dpf. Scale bar: 10 μm.

Acknowledgments

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