|
Fig. 4 Quantification of acute toxicity of PMF in adult zebrafish. (A) 96-h probit analysis showing dose-dependent mortality following exposure to increasing concentrations of PMF. (B) Representative images illustrating acute toxicity effects of PMF. Zebrafish exposed to control (0 mg/mL), 24 mg/mL, 26 mg/mL, 28 mg/mL, and 30 mg/mL PMF displayed normal morphology and behaviour after 96 h. In contrast, exposure to higher concentrations (32–44 mg/mL) induced clear toxic effects characterized by reduced locomotive activity, spinal curvature, discoloration, lethargy, internal hemorrhaging (red arrows), and mortality observed between 24 and 72 h post-treatment.
Reprinted from Molecular and Cellular Endocrinology, , Vohra, M.S., Ahmad, B., Taylor, E.R., Benchoula, K., Fong, I.L., Parhar, I.S., Ogawa, S., Serpell, C.J., Wong, E.H., 5,7,3',4',5'-Pentamethoxyflavone (PMF) Exhibits Anti-Obesity and Neuroprotective Effects in an Obese Zebrafish Model, 112554112554, Copyright (2025) with permission from Elsevier. Full text @ Mol. Cell. Endocrinol.