Figure 4

Figure 4

Pharmacological inhibition of nuclear factor kappa B (NF-κB) abolishes the protection of Samhd1-deficient zebrafish larvae to infection with Salmonella enterica serovar Typhimurium (STM). Zebrafish one-cell embryos were injected with gRNA (samhd1 or crSTD)/Cas9 complexes, dechorionated at 24 h, and treated by bath immersion with 10 nM BAY11-7082 (BAY) (A) or with 10 μM baricitinib (B). Drugs were renewed every day for the next 6 days. Controls were incubated with 0.1% dimethyl sulfoxide (DMSO). Larvae were infected at 2 days post-fertilization via the yolk sac with STM at a multiplicity of infection (MOI) of 10, with the number of surviving larvae counted daily during the next 5 days. A total number of >100 specimens/treatment. P-values were calculated using a long-range test. **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001.