Figure 3

Figure 3

Samhd1 deficiency leads to an activated inflammatory state. (A–F) Transcript levels of nfkb1(A), il1b(B), isg15(C), tnfα(D), ifng1r(E), and cxcl8a(F) in larvae injected with gRNA (samhd1 or STD)/Cas9 complexes infected or not with Salmonella enterica serovar Typhimurium (STM) for 24 h assayed using reverse transcription quantitative PCR (RT-qPCR). Bars show the mean ± SEM of three independent experiments, each with 25 pooled larvae. Data are represented as fold change from control. P-values were calculated using one-way ANOVA. ns, not significant. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001.