FIGURE 4

FIGURE 4

Upregulation of SASP factors in Müller glia after light and NMDA damage. Bulk RNAseq was performed on gfap⁺ Müller glia after either NMDA or light damage compared to undamaged controls. (A–D) Volcano plots showing differentially expressed SASP factors at the indicated times post light damage. (E–H) Volcano plots showing differentially expressed SASP factors at the indicated times post NMDA damage. Dashed lines represent values with a |log₂FC| > 2 and p-values <10⁻⁶. Top significantly upregulated factors are labeled for each time point. (I) Heatmap showing enrichment of SASP factors at the indicated times post light or NMDA damage. SASP factors were included in the heatmap if they had a log₂FC > 5 with p-values <10⁻⁶ for at least one time point; bolded genes are part of overlapping set of SRRFs.