ZFIN Image: Klem et al., 2025, Fig. 5.

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Figure Caption

Fig. 5.

Ethanol exposure changes shape of oral ectoderm expression domain in bmp4^−/−;smad5^−/− zebrafish embryos. (A-D) Whole-mount confocal images of untreated (top panels) or ethanol-treated (bottom panels) wild-type (left) or bmp4;smad5;sox17:EGFP (right) zebrafish embryos fluorescence labeled for fgf8a (magenta) gene expression at 36 hpf. Views are lateral, with anterior to the left. Scale bar: 100 μm. The endoderm is labeled with GFP (green). Arrows in A-C indicate normal expression of fgf8a in the oral ectoderm of untreated wild-type and bmp4^−/−;smad5^−/− embryo as well as ethanol-treated wild-type embryos. Arrowhead in D indicates that the domain of fgf8a expression in ethanol-treated bmp4^−/−;smad5^−/− embryos is expanded anteriorly (n=7 embryos per group). Panel insets are magnified (10×) views of the indicated fgf8a expression domains.

Acknowledgments

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