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Fig. 7 Downregulation of ezh2 reduces zebrafish behavioural activity (A) Under LD conditions, the locomotor activity of ezh2−/− mutant zebrafish and ezh2 morpholino-treated 4 dpf larvae (n = 16) was monitored for 3 consecutive days. The line plot represents the average swimming distance per 10 min over 3 days for each group, with shaded areas showing confidence intervals. (B) Average swimming distance over 3 days for different groups, analysed via t tests for statistical significance. (C–D) Average daytime and nighttime swimming distances over 3 days for different groups, with t-test significance analysis. (E) Proportions of duration, duration, and duration of total activity over 3 days; reduced ezh2 increased the proportion of nonstationary activity. (F) Under DD conditions, the locomotor activity of ezh2−/− mutant zebrafish and ezh2 morpholino-treated 4 dpf larvae (n = 16) was monitored for 3 consecutive days. The line plot represents the average swimming distance per 10 min, with shaded areas indicating confidence intervals. (G) Polynomial fitting of the swimming distance curves for the different groups. (H) Average swimming distance over 3 days for different groups, with t-test significance analysis. (I) JTK-Cycle periodicity analysis of DD data revealed significant increases in period, reduced amplitude expression, and delayed phase shift in both groups. (t-test significance: ns, p > 0.05; ∗, p < 0.05; ∗∗, p < 0.01; ∗∗∗, p < 0.001; ∗∗∗∗, p < 0.0001).