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Fig. 2 Anterior protrusion of the swim bladder in hoxc6a mutant fish showing the absence of the os suspensorium and the malformed tripus. (a–f) Representative micro-CT scan images of the anterior vertebrae in wild-type (n = 4) and hoxc6a homozygous adult fish (n = 4). In the images, the os suspensorium is indicated by the yellow arrowhead, while the tripus is indicated by the white arrowhead in wild-type fish. A 3D movie is available in Movie S6. (g) Visualization of the swim bladder (depicted in red) and the surrounding bones in hoxc6a mutant fish (n = 4). The presumptive anterior edge of the swim bladder in normal adult fish is indicated by the yellow dashed line. The 3D movies are available with Movies S7 and S8. Furthermore, a 2D movie illustrating the outline of the swim bladder is provided in Movie S9 (sagittal view). Scale bars: 1000 μm.