Fig. 4

Fig. 4 MZ ptprz1b and MZ mdka mutants exhibit delayed neural keel convergence. (A-B) Whole-mount in situ RNA hybridization of WT, MZ ptprz1b and MZ mdka embryos at 10 and 12 hpf, and MZ mdkb, MZ mdka; MZ mdkb, MZ mdka; MZ ptn and MZ mdka; MZ mdkb; MZ ptn mutant embryos at 12 hpf. The maximum width of forming r3 and r5 is measured (white bar) using egr2b as a marker. Forebrain, mid-hindbrain boundary and somites are labelled by emx1, eng2a and myoD as landmarks, respectively. (C-F) Quantification of maximum width of r3 at 10 hpf (C) and r3 and r5 at 12 hpf (D-E). Data are presented as scattered dots. Mean ± SD of each dataset is indicated by line and error bar. Statistical comparison was performed between WT (N = 19, 10 hpf; N = 10, 12 hpf) and MZ ptprz1b (N = 10, 10 hpf; N = 8, 12 hpf) or MZ mdka (N = 13, 10 hpf; N = 10, 12 hpf) and between WT (N = 20, 12 hpf) and MZ mdkb (N = 15, 12 hpf), or MZ mdka; MZ mdkb (N = 15, 12 hpf) or MZ mdka; MZ ptn (N = 30, 12 hpf) or MZ mdka; MZ mdkb; MZ ptn (N = 20, 12 hpf) on Estimation Stats (https://www.estimationstats.com). P values calculated by two-sided permutation t-tests under a CI of 95% are shown in (F). Asterisks indicate statistical significance (P < 0.05).