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Fig. 3 Nec-1 mitigates ROS production in PTZ-induced epileptic zebrafish larvae. A The ROS levels were measured by image analysis and fluorescence microscope. B Individual zebrafish fluorescence intensity was quantified using an ImageJ program. Evaluation of ROS levels using DCFH-DA fluorescent probe in zebrafish larvae at 7 dpf. Exposure to PTZ resulted in a significant elevation in ROS levels, whereas prior administration of Nec-1 (15 µM) effectively mitigated ROS generation. The images were acquired with an inverted fluorescent microscope (Optika IM3, Italy) with a magnification of × 10. Results are expressed as mean ± SD, each performed in triplicate (n = 15). Data were analyzed by one-way ANOVA, followed by Tukey’s post hoc test